Wang Cang, Wu Jian, Xu Zhi-Kang
Department of Chemistry, Zhejiang University, Hangzhou 310027, P. R. China.
Macromol Rapid Commun. 2010 Jun 16;31(12):1078-82. doi: 10.1002/marc.200900866. Epub 2010 Mar 24.
To biologically mimic the carbohydrate-protein interactions in artificial systems, one of the challenges is to construct a glycosylated surface with a high glycosyl density to yield a notable 'glycoside cluster effect'. A novel strategy is presented for high density glycosylation of the surface of a microporous poly(propylene) membrane (MPPM) by click chemistry. It is promising that the surface glycosyl density can be well controlled over a wide range and the maximum value is over 10 µmol · cm(-2) . The recognition capability of these glycosylated MPPMs to lectins indicates the occurrence of the 'glycoside cluster effect' when the glycosyl density on the membrane surface exceeds 0.20 µmol · cm(-2) .
为了在人工系统中从生物学角度模拟碳水化合物 - 蛋白质相互作用，其中一项挑战是构建具有高糖基密度的糖基化表面，以产生显著的“糖苷簇效应”。本文提出了一种通过点击化学对微孔聚丙烯膜（MPPM）表面进行高密度糖基化的新策略。有望在很宽的范围内很好地控制表面糖基密度，其最大值超过10 μmol·cm⁻² 。这些糖基化MPPM对凝集素的识别能力表明，当膜表面糖基密度超过0.20 μmol·cm⁻² 时，会发生“糖苷簇效应”。
