MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Yu Gu Road 20#, Hangzhou, Zhejiang Province, China.
Colloids Surf B Biointerfaces. 2011 Jun 15;85(1):19-25. doi: 10.1016/j.colsurfb.2010.10.030. Epub 2010 Nov 11.
Carbohydrates (saccharides) are ubiquitous on the extracellular surface of living cells and mediate a myriad of biological recognition and signaling processes. Carbohydrate decoration of polymer surfaces with covalent attachment of saccharides offers a new realm of opportunities to mimic cellular events such as protein recognition and binding. We describe the carbohydrate decoration (surface glycosylation) of poly(2-hydroxyethyl methacrylate)-grafted microporous polypropylene membranes (poly(HEMA)-g-MPPMs) with mono- and disaccharides. Galactose, lactose, glucose, and maltose were covalently attached on the surfaces of poly(HEMA)-g-MPPMs and were compared in detail. The process was verified by solid state (13)C NMR spectra. Membranes with high binding degree (BD) of saccharide ligands on the surfaces were facilely prepared from poly(HEMA)-g-MPPMs with high grafting degree (GD) of poly(HEMA). For poly(HEMA)-g-MPPM with the same GD of poly(HEMA), the BD of disaccharides is lower than that of monosaccharides and the disaccharide-decorated MPPMs are more hydrophilic than the monosaccharide-decorated ones. The carbohydrate-decorated MPPMs prepared from galactose, lactose, glucose, and maltose (denoted as MPPM-Gal, MPPM-Lac, MPPM-Glc and MPPM-Mal, respectively) recognize and adsorb specifically one of the two lectins, concanavalin A (Con A) and peanut agglutinin (PNA). As the BD of saccharide increases, the "glycoside cluster effect" plays a primary role in lectin adsorption. MPPM-Lac has enhanced affinity to PNA as compared with MPPM-Gal having similar BD of saccharide., on the other hand, MPPM-Mal shows no enhanced affinity to Con A in comparison with MPPM-Glc as the BD of saccharide is above 0.9 μmol/cm(2), where the "glycoside cluster effect" occurs.
碳水化合物（糖）普遍存在于活细胞的细胞外表面，介导了无数的生物识别和信号转导过程。通过共价连接糖将碳水化合物修饰在聚合物表面上，为模拟细胞事件（如蛋白质识别和结合）提供了新的机会。我们描述了单糖和二糖对聚（2-羟乙基甲基丙烯酸酯）接枝微孔聚丙烯膜（poly（HEMA）-g-MPPMs）的碳水化合物修饰（表面糖基化）。半乳糖、乳糖、葡萄糖和麦芽糖通过共价键连接到 poly（HEMA）-g-MPPMs 表面，并进行了详细比较。该过程通过固态（13）C NMR 谱进行了验证。从具有高接枝度（GD）的 poly（HEMA）-g-MPPMs 制备了具有高糖配体结合度（BD）的表面糖基化的膜。对于具有相同 GD 的 poly（HEMA）-g-MPPM，二糖的 BD 低于单糖，并且二糖修饰的 MPPM 比单糖修饰的 MPPM 更亲水。从半乳糖、乳糖、葡萄糖和麦芽糖制备的碳水化合物修饰的 MPPM（分别表示为 MPPM-Gal、MPPM-Lac、MPPM-Glc 和 MPPM-Mal）特异性地识别和吸附两种凝集素中的一种，伴刀豆球蛋白 A（Con A）和花生凝集素（PNA）。随着糖的 BD 增加，“糖苷簇效应”在凝集素吸附中起主要作用。与具有相似糖 BD 的 MPPM-Gal 相比，MPPM-Lac 对 PNA 具有增强的亲和力。另一方面，当糖的 BD 高于 0.9 μmol/cm（2）时，即发生“糖苷簇效应”，与具有相似糖 BD 的 MPPM-Glc 相比，MPPM-Mal 对 Con A 没有增强的亲和力。
