Beliasova N A, Prokulevich V A, Fomichev Iu K
Mol Gen Mikrobiol Virusol. 1990 Jan(1):14-8.
The bacteriophage P1 Cm clr100 lysogenises the bacteria E. chrysanthemi, E. aroideae, E. atroseptica being localized in the cytoplasm, replicating but causing no cell lysis. The prophage induction results in transformation of the lysogenic bacteria E. chrysanthemi into nonviable filamentous cells. However, a portion of cells gets rid of the prophage and gives rise to normal heritage inheritors permitting to use the bacteriophage as an efficient vehicle for introducing the transposons into the chromosome of E. chrysanthemi. The transposon Tn9 has been found to insert into the different chromosomal sites causing no inactivation of the genes, while the transposition of Tn5 from the bacteriophage P1::Tn5Cmclr100 induces the different mutations with the frequency up to 3%. The bacteriophage P1Cmclr100 may also serve a tool for construction of the homology regions in the chromosome of E. chrysanthemi and Flac+ plasmid for further construction of Hfr-type donors.
噬菌体P1 Cm clr100可使菊欧文氏菌、天南星科欧文氏菌、黑胫欧文氏菌溶源化，其定位于细胞质中，进行复制但不引起细胞裂解。原噬菌体诱导可使溶源化的菊欧文氏菌转变为无活力的丝状细胞。然而，一部分细胞可去除原噬菌体并产生正常的遗传后代，从而使得该噬菌体可作为一种有效的载体，用于将转座子导入菊欧文氏菌的染色体。已发现转座子Tn9可插入不同的染色体位点，且不会导致基因失活，而来自噬菌体P1::Tn5Cmclr100的Tn5转座会诱导不同的突变，频率高达3%。噬菌体P1Cmclr100还可作为一种工具，用于在菊欧文氏菌染色体和Flac+质粒中构建同源区域，以便进一步构建Hfr型供体。
