Didyk O A, Mukvich N S, Iagovdik M V, Kishko Ia G
Mol Gen Mikrobiol Virusol. 1990 Mar(3):24-6.
The present communication deals with the genetic mapping of the temperate bacteriophage 59 immunity region. Morphological mutants of the c-type of the temperate bacteriophage 59 were isolated after UV-treatment of the phage. Complementation analysis of 36 c-mutants has shown that the bacteriophage 59 c-region consists of at least four genes responsible for the establishment of lysogenization. The two-factor crosses were used to determine the mutual positions (order and distances) of the c-mutations. The order of the c-genes is C1--C3--C2--C4. The three c-genes are localized in a short region of the phage 59 chromosome, but the fourth gene is located in the separate locus.
本通讯涉及温和噬菌体59免疫区域的基因定位。对温和噬菌体59进行紫外线处理后，分离出了c型形态突变体。对36个c突变体的互补分析表明，噬菌体59的c区域至少由四个负责溶原化建立的基因组成。利用双因子杂交来确定c突变的相互位置（顺序和距离）。c基因的顺序是C1--C3--C2--C4。其中三个c基因位于噬菌体59染色体的一个短区域，但第四个基因位于单独的位点。
