Method to identify genomic targets of DNA binding proteins.
作者信息
Sompayrac L, Danna K J
机构信息
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347.
出版信息
Proc Natl Acad Sci U S A. 1990 May;87(9):3274-8. doi: 10.1073/pnas.87.9.3274.
Abstract
We have devised a cyclical immunoprecipitation protocol that can be used to identify and clone a specific DNA sequence that is recognized by a DNA binding protein, even if that sequence is present in only one copy in the genome of a mammal. As an example, we have used this procedure to purify mouse genomic sequences to which the simian virus 40 tumor (T) antigen binds.
摘要
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