鉴定DNA结合蛋白基因组靶点的方法。
Method to identify genomic targets of DNA binding proteins.
作者信息
Sompayrac L, Danna K J
机构信息
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347.
出版信息
Proc Natl Acad Sci U S A. 1990 May;87(9):3274-8. doi: 10.1073/pnas.87.9.3274.
Abstract
We have devised a cyclical immunoprecipitation protocol that can be used to identify and clone a specific DNA sequence that is recognized by a DNA binding protein, even if that sequence is present in only one copy in the genome of a mammal. As an example, we have used this procedure to purify mouse genomic sequences to which the simian virus 40 tumor (T) antigen binds.
摘要
我们设计了一种循环免疫沉淀方案，可用于识别和克隆被DNA结合蛋白识别的特定DNA序列，即使该序列在哺乳动物基因组中仅以单拷贝形式存在。例如，我们已使用此方法纯化猿猴病毒40肿瘤（T）抗原所结合的小鼠基因组序列。
Zotero 插件