Department of Food and Applied Technology, Hungkuang University, 34 Chung-Chie Road, Shalu County, Taichung Hsien 43302, Taiwan.
J Agric Food Chem. 2011 Jun 22;59(12):6730-9. doi: 10.1021/jf200770n. Epub 2011 May 18.
Fermented soybean liquid (FSL) has been well cited for its broad spectrum of biological effects, yet its documented gastropeptic ulcer (GPU) ameliorating effect is still lacking. It was hypothesized that to avoid the injury exerted by gastric fluid, HP has to be sheltered in chyme emulsions immediately on infection. The HP urease (HPU) and the acidic proton pump (PP) may act as the "two-point pH modulator" to maintain an optimum pH between 6 and 7, and FSL is able to destroy such a modulating mechanism. FSL exhibited higher contents of isoflavonoids (2.5-17.3-fold) and essential amino acids (1.5-4.0-fold) than the nonfermented. FSL administered at 1 g/20 mL tid for 3 months eradicated Helicobacter pylori (HP) by 82% in 37 volunteers having GPU (p < 0.20); simultaneously, the plasma conjugated diene and TBARs levels were significantly resumed (p < 0.05). Kinetic analysis based on the conventional "urease theory" revealed that a cluster of 2.0 × 10(9) of HP cells is required for a single attack in the gastric lumen at pH 1.0-2.5. To verify the hypothesis, chyme-shelter testing was conducted in artificial gastric fluid (pH 2.4 ± 0.20). Results showed the HP cell viability was time- and size-dependent. At 20 min of contact time, the viability was 100, 4.2, 31.4, 43.3, 57.2, and 82.6%, respectively, in intact, dispersed, and particulate chymes (mesh sizes 80, 60, 40, and 20). The corresponding data became 96.2, 0.0, 14.5, 18.5, 21.3, and 28.6%, respectively, at a contact time of 40 min. Conclusively, the kinetic analysis and the chyme-shelter testing revealed that direct infection by bare HP cells is unlikely in real status. FSL is beneficial to GPU most probably due to its ability to raise blood alkalinity levels, destroying the PP and its ROS suppressing effect.
发酵大豆液（FSL）因其广泛的生物学效应而备受赞誉，但它在治疗胃溃汤（GPU）方面的作用仍缺乏相关记录。据推测，为了避免胃液造成的损伤，幽门螺杆菌（HP）必须在感染后立即被包裹在食糜乳液中。HP 的尿素酶（HPU）和酸性质子泵（PP）可能起到“两点 pH 调节剂”的作用，将 pH 值维持在 6 到 7 之间，而 FSL 能够破坏这种调节机制。与非发酵大豆液相比，FSL 中的异黄酮（2.5-17.3 倍）和必需氨基酸（1.5-4.0 倍）含量更高。在 37 名患有 GPU 的志愿者中，FSL（1g/20mL，每日 3 次）治疗 3 个月后，HP 根除率达到 82%（p<0.020）；同时，血浆共轭二烯和 TBARs 水平也显著恢复（p<0.05）。基于传统“尿素酶理论”的动力学分析表明，在 pH 值为 1.0-2.5 的胃腔中，需要一簇 2.0×10(9)个 HP 细胞才能对单个攻击做出反应。为了验证这一假设，在人工胃液（pH 2.4±0.20）中进行了食糜保护测试。结果表明，HP 细胞的存活率与时间和大小有关。在 20 分钟的接触时间内，完整、分散和颗粒状食糜（网孔尺寸分别为 80、60、40 和 20）中的细胞存活率分别为 100%、4.2%、31.4%、43.3%、57.2%和 82.6%；而接触时间为 40 分钟时，相应数据分别为 96.2%、0.0%、14.5%、18.5%、21.3%和 28.6%。总之，动力学分析和食糜保护测试表明，在实际情况下，裸 HP 细胞的直接感染不太可能发生。FSL 对 GPU 有益，可能是因为它能够提高血液的碱度水平，破坏 PP 及其对 ROS 的抑制作用。
