精索静脉曲张对精子功能和精液氧化应激的影响。
Effect of varicocele on sperm function and semen oxidative stress.
机构信息
Department of Surgery, Division of Urology, Human Reproduction Section, Sao Paulo Federal University, Brazil.
出版信息
BJU Int. 2012 Jan;109(2):259-65. doi: 10.1111/j.1464-410X.2011.10240.x. Epub 2011 May 18.
OBJECTIVE
To assess the effect of varicocele on sperm DNA integrity, mitochondrial activity, lipid peroxidation and acrosome integrity.
PATIENTS AND METHODS
In all, 30 patients with a clinically diagnosed varicocele of grade II or III and 32 men without a varicocele were evaluated for sperm DNA fragmentation (comet assay), mitochondrial activity (3,3'-diaminobenzidine assay), lipid peroxidation (malondialdehyde) and acrosome integrity (fluorescent probe labelled peanut agglutinin).
RESULTS
The varicocele group showed fewer spermatozoa with intact DNA (grade II, P= 0.040), more cells with inactive mitochondria (class III, P= 0.001), fewer cells with active mitochondria (class I, P= 0.005) and fewer spermatozoa with intact acrosomes (P < 0.001). Finally, no significant differences were observed in lipid peroxidation levels.
CONCLUSION
Men with varicocele showed an increase in sperm DNA fragmentation and a reduction in mitochondrial activity and acrosome integrity. However, lipid peroxidation levels remained unchanged.
目的
评估精索静脉曲张对精子 DNA 完整性、线粒体活性、脂质过氧化和顶体完整性的影响。
患者与方法
共评估了 30 例临床诊断为 II 级或 III 级精索静脉曲张患者和 32 例无精索静脉曲张的男性的精子 DNA 碎片化（彗星试验）、线粒体活性（3,3'-二氨基联苯胺试验）、脂质过氧化（丙二醛）和顶体完整性（荧光探针标记的花生凝集素）。
结果
精索静脉曲张组具有完整 DNA 的精子数量较少（II 级，P=0.040），无活性线粒体的细胞较多（III 级，P=0.001），活性线粒体的细胞较少（I 级，P=0.005），顶体完整的精子较少（P<0.001）。最后，脂质过氧化水平没有观察到显著差异。
结论
精索静脉曲张患者的精子 DNA 碎片化增加，线粒体活性和顶体完整性降低。然而，脂质过氧化水平保持不变。
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