精液氧化应激对精子质量的影响：来自越南的初步结果。

Impact of semen oxidative stress on sperm quality: initial results from Vietnam.

机构信息

Center for Reproductive Endocrinology and Infertility, Hue University of Medicine and Pharmacy, Hue University, Vietnam.

Department of Obstetrics and Gynecology, Hue University of Medicine and Pharmacy, Hue University, Vietnam.

出版信息

J Int Med Res. 2023 Aug;51(8):3000605231188655. doi: 10.1177/03000605231188655.

DOI:10.1177/03000605231188655

PMID:37572034

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10423449/

Abstract

OBJECTIVES

This study aimed to determine the relationship between oxidative stress (OS) measured by the oxidation-reduction potential (ORP) and the results of semen analysis among men from infertile couples.

METHODS

This cross-sectional study included 166 men from infertile couples, determined according to the World Health Organization guidelines. The general characteristics, semen analysis, sperm chromatin dispersion assay, and ORP of all subjects were evaluated and analyzed statistically.

RESULTS

Among 166 men from infertile couples, individuals with OS had a significantly higher DNA fragmentation index (DFI) than men without OS (22.37% ± 11.67% vs. 17.98% ± 8.98%). The sperm concentration, total sperm count, motility rate, and normal morphology were negatively correlated, while and an abnormal head and neck-tail were positively correlated with ORP. There was also a positive association between the DFI and OS level. The optimal ORP threshold for determining sperm quality was 0.77 mV/10 sperm/mL (sensitivity, 50.4%; specificity, 93.5%; positive predictive value, 52.9%; negative predictive value, 32.3%).

CONCLUSIONS

Determining the ORP suggests that OS has an adverse effect on the total sperm count, sperm motility, sperm concentration, morphology, vitality, and DNA fragmentation index.

摘要

目的

本研究旨在确定氧化应激（OS）与氧化还原电位（ORP）与不孕夫妇男性精液分析结果之间的关系。

方法

本横断面研究纳入了根据世界卫生组织（WHO）指南确定的 166 名不孕夫妇男性。对所有受试者的一般特征、精液分析、精子染色质扩散试验和 ORP 进行评估和统计学分析。

结果

在 166 名不孕夫妇男性中，有 OS 的个体的 DNA 碎片化指数（DFI）明显高于无 OS 的个体（22.37%±11.67%比 17.98%±8.98%）。精子浓度、总精子计数、活力和正常形态与 ORP 呈负相关，而异常头颈部-尾部与 ORP 呈正相关。DFI 与 OS 水平也呈正相关。确定精子质量的最佳 ORP 阈值为 0.77 mV/10 个精子/mL（灵敏度为 50.4%；特异性为 93.5%；阳性预测值为 52.9%；阴性预测值为 32.3%）。