Urology and Nephrology Center Physiology Department Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
BJU Int. 2011 Aug;108(4):612-8. doi: 10.1111/j.1464-410X.2010.09943.x. Epub 2011 May 18.
What's known on the subject? and What does the study add? Renal ischaemia/reperfusion (I/R) injury is an inevitable consequence of kidney transplantation. It contributes to delayed graft function (DGF), acute renal failure and graft rejection. The present study investigates for the first time the impact of a combination of L-arginine and alpha tocopherol on the renal ischemia/reperfusion injury in a rodent model of kidney transplantation. We found that concomitant administration of L-arginine and α-tocopherol has a more protective effect and synergistic antioxidant effect on ischaemia/reperfusion injury in transplanted rat kidneys.
To investigate the role of L-arginine and α-tocopherol in ischaemia/reperfusion injury in a kidney transplanted rat model.
In total, 40 male Sprague-Dawley rats subjected to renal transplantation received FK506 (tacrolimus) to overcome early acute rejection episodes. Animals were divided randomly into four groups (ten rats each). Group I were treated with FK506 (2 mg/kg/bw/day) and served as the control group. Group II were treated with L-arginine 300 mg/kg/bw. Group III were treated with α-tocopherol 30 mg/kg/bw. Group IV were treated with L-arginine and α-tocopherol. Urine and blood samples were taken at 0 (before operation), 2, 7 and 14 days post-transplantation for estimation of urine sodium, creatinine, fractional excretion of sodium, serum creatinine, sodium and blood urea nitrogen. Histological examination and measurement of malondialdehyde in kidney tissues were also performed.
Serum creatinine and blood urea nitrogen significantly decreased in L-arginine and α-tocopherol, as well as combination groups, compared to the control group. Malondialdehyde was significantly decreased in the combination group compared to L-arginine and α-tocopherol alone. Histological examination of the control group showed that acute tubular necrosis was markedly decreased in transplanted kidneys treated with a combination of both L-arginine and α-tocopherol.
Concomitant administration of l-arginine and α-tocopherol has a more protective effect and synergistic antioxidant effect on ischaemia/reperfusion injury in transplanted rat kidneys.
研究左旋精氨酸和α-生育酚在肾移植大鼠模型中缺血/再灌注损伤中的作用。
共 40 只雄性 Sprague-Dawley 大鼠接受肾移植，接受 FK506（他克莫司）以克服早期急性排斥反应。动物随机分为四组（每组 10 只）。第 I 组给予 FK506（2mg/kg/bw/天）作为对照组。第 II 组给予左旋精氨酸 300mg/kg/bw。第 III 组给予α-生育酚 30mg/kg/bw。第 IV 组给予左旋精氨酸和α-生育酚。在移植后 0（手术前）、2、7 和 14 天采集尿液和血液样本，用于估计尿钠、肌酐、钠排泄分数、血清肌酐、钠和血尿素氮。还进行了组织学检查和肾组织丙二醛测量。
与对照组相比，左旋精氨酸和α-生育酚以及联合组的血清肌酐和血尿素氮明显降低。与单独使用左旋精氨酸和α-生育酚相比，联合组的丙二醛明显降低。对照组的组织学检查显示，在联合使用左旋精氨酸和α-生育酚的移植肾脏中，急性肾小管坏死明显减少。
左旋精氨酸和α-生育酚联合应用对肾移植大鼠缺血/再灌注损伤具有更强的保护作用和协同抗氧化作用。
