Departamento de Investigaciones, Instituto de Nefrología Dr. Abelardo Buch, Ave. 26 y Ave. Rancho Boyeros, La Habana, Cuba.
Nefrologia. 2011;31(4):464-70. doi: 10.3265/Nefrologia.pre2011.May.10873.
Ischaemia-reperfusion is one of the main causes of kidney complications. The most frequent lesion is acute tubular necrosis. Ozone oxidative preconditioning exerts a modulatory effect of redox state of renal cells in models of ischaemia-reperfusion, by stimulating endogenous antioxidant mechanisms. Similar results have been obtained in more recent studies using ischaemic postconditioning.
To evaluate the effect of ozone oxidative postconditioning on renal function and morphology in an ischaemia-reperfusion rat model.
We used forty female Wistar rats weighing between 150g-200g randomly divided into 4 groups (negative control, positive control, oxygen and ozone). The groups: positive control, oxygen and ozone were subjected to 60 minutes of ischaemia and 10 days of reperfusion. During reperfusion, the oxygen group was given 26mg/kg body weight of oxygen, and the ozone group 0.5mg/kg body weight of ozone, rectally. At the end of the experiment urine and blood samples were taken for renal function tests and kidneys were removed for histological study.
The ozone group showed no significant differences for filtration fraction and proteinuria compared to the negative control group. The glomerular filtrate rate, renal plasma flow and creatinine showed a slight improvement in comparison with oxygen and positive control groups. The ozone group showed significantly less overall histological damage than the positive control and oxygen groups.
Ozone postconditioning showed to have a protective effect in preserving renal function and morphology.
缺血再灌注是肾脏并发症的主要原因之一。最常见的病变是急性肾小管坏死。臭氧氧化预处理通过刺激内源性抗氧化机制，对缺血再灌注模型中的肾细胞氧化还原状态发挥调节作用。在最近使用缺血后处理的研究中也得到了类似的结果。
评估臭氧氧化后处理对缺血再灌注大鼠模型肾功能和形态的影响。
我们使用了 40 只体重在 150g-200g 之间的雌性 Wistar 大鼠，随机分为 4 组（阴性对照组、阳性对照组、氧气组和臭氧组）。阳性对照组、氧气组和臭氧组进行 60 分钟的缺血和 10 天的再灌注。在再灌注期间，氧气组给予 26mg/kg 体重的氧气，臭氧组给予 0.5mg/kg 体重的臭氧，直肠给药。实验结束时，采集尿液和血液样本进行肾功能检查，并取出肾脏进行组织学研究。
与阴性对照组相比，臭氧组的滤过分数和蛋白尿没有显著差异。与氧气组和阳性对照组相比，肾小球滤过率、肾血浆流量和肌酐略有改善。臭氧组的整体组织损伤明显低于阳性对照组和氧气组。
臭氧后处理在保护肾功能和形态方面显示出保护作用。
