School of EECS, Korea Advanced Institute of Science and Technology, 335 Gwahakro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Biosens Bioelectron. 2011 Jul 15;26(11):4591-5. doi: 10.1016/j.bios.2011.04.033. Epub 2011 Apr 28.
This paper describes a sensor for label-free, fully electrical detection of DNA hybridization based on capacitive changes in the electrode-electrolyte interface. The sensor measures capacitive changes in real time according to a charging-discharging principle that is limited by the hysteresis window. In addition, a novel autonomous searching technique, which exclusively monitors desorption-free hybridized electrodes among electrode arrays, enhances the performance of the sensor compared with conventional capacitive measurement. The sensor system achieves a detection range of 80 dB. The integrated circuit sensor is fabricated with a 0.35 μm CMOS process. The proposed sensor offers rapid, robust and inexpensive measurement of capacitance with highly integrated detection circuitry. It also facilitates quantitative evaluations of molecular densities on a chip with distinctive impedance variations by monitoring desorption-free hybridized electrodes. Our electrical biosensor has great potential for use with bio analytical tools and point-of-care diagnosis.
本文介绍了一种基于电极-电解质界面电容变化的无标记、全电化 DNA 杂交传感器。该传感器根据受滞后窗口限制的充放电原理实时测量电容变化。此外，一种新颖的自主搜索技术，专门监测电极阵列中无脱附杂交的电极，与传统电容测量相比，提高了传感器的性能。该传感器系统实现了 80dB 的检测范围。集成电路传感器采用 0.35μm CMOS 工艺制造。所提出的传感器具有快速、稳健和低成本的电容测量功能，具有高度集成的检测电路。通过监测无脱附杂交的电极，它还可以通过监测无脱附杂交的电极，以独特的阻抗变化来实现对芯片上分子密度的定量评估。我们的电化学生物传感器在生物分析工具和即时诊断方面具有很大的应用潜力。
