在表皮生长因子刺激的成纤维细胞中，1,2 - 二酰基甘油的持续增加先于DNA合成。磷脂酰胆碱水解受刺激的证据。
Sustained increase in 1,2-diacylglycerol precedes DNA synthesis in epidermal-growth-factor-stimulated fibroblasts. Evidence for stimulated phosphatidylcholine hydrolysis.
作者信息
Wright T M, Shin H S, Raben D M
机构信息
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
出版信息
Biochem J. 1990 Apr 15;267(2):501-7. doi: 10.1042/bj2670501.
Abstract
A property common to many growth factors is that they must be present for several hours before the commitment to DNA synthesis and cell division occurs. The intracellular signals that are relevant during this period are poorly defined. We examined the formation of 1,2-diacylglycerol in IIC9 fibroblasts after stimulation with epidermal growth factor (EGF), and found that the mass of this lipid remained elevated for at least four hours. The concentration-dependence of EGF-stimulated 1,2-diacylglycerol production and [3H]thymidine incorporation were similar. Studies of phospholipid metabolism strongly suggested that phosphatidylcholine was the source of the 1,2-diacylglycerol generated in response to EGF. EGF did not stimulate the hydrolysis of other phospholipids, including the phosphoinositides, nor did it increase synthesis de novo of 1,2-diacylglycerol. This pattern of sustained 1,2-diacylglycerol formation from phosphatidylcholine may be important in the mitogenic signalling of EGF and potentially other growth factors.
摘要
许多生长因子的一个共同特性是，在细胞开始进行DNA合成和细胞分裂之前，它们必须存在数小时。在此期间相关的细胞内信号还不清楚。我们检测了表皮生长因子（EGF）刺激后IIC9成纤维细胞中1,2 - 二酰基甘油的形成，发现这种脂质的量至少在4小时内持续升高。EGF刺激的1,2 - 二酰基甘油生成和[³H]胸苷掺入的浓度依赖性相似。磷脂代谢研究强烈表明，磷脂酰胆碱是响应EGF产生的1,2 - 二酰基甘油的来源。EGF不刺激其他磷脂（包括磷酸肌醇）的水解，也不增加1,2 - 二酰基甘油的从头合成。这种由磷脂酰胆碱持续形成1,2 - 二酰基甘油的模式可能在EGF以及其他潜在生长因子的促有丝分裂信号传导中起重要作用。
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