用原位杂交组织化学法对大鼠基底前脑神经生长因子受体mRNA进行定位。
Localization of nerve growth factor receptor mRNA in the rat basal forebrain with in situ hybridization histochemistry.
作者信息
Springer J E, Robbins E, Meyer S, Baldino F, Lewis M E
机构信息
Department of Neurology, Hahnemann Institute of Neuroscience, Hahnemann University School of Medicine, Philadelphia, Pennsylvania 19102-1192.
出版信息
Cell Mol Neurobiol. 1990 Mar;10(1):33-9. doi: 10.1007/BF00733633.
Abstract
- In situ hybridization histochemistry was used to localize nerve growth factor receptor (NGFR) mRNA in the adult rat basal forebrain. 2. In emulsion-dipped sections 35S-labeled RNA antisense probes produced a high density of silver grains over cells located in the medial septum, vertical and horizontal limbs of the diagonal band of Broca, and nucleus basalis. 3. This distribution of NGFR mRNA overlaps with the distribution of NGFR protein localized using immunocytochemical techniques. 4. No hybridization signal was detected when sections were hybridized with a 35S-labeled RNA sense (control) probe. 5. We suggest that NGFRs are synthesized in these basal forebrain nuclei and transported to terminal areas where NGF is thought to be bound and internalized, an initial step in the many actions of this neurotrophic factor.
摘要
- 采用原位杂交组织化学方法在成年大鼠基底前脑定位神经生长因子受体（NGFR）mRNA。2. 在浸有乳胶的切片中，35S标记的RNA反义探针在位于内侧隔核、布罗卡斜带垂直和水平支以及基底核的细胞上产生了高密度的银颗粒。3. NGFR mRNA的这种分布与使用免疫细胞化学技术定位的NGFR蛋白的分布重叠。4. 当切片与35S标记的RNA正义（对照）探针杂交时未检测到杂交信号。5. 我们认为NGFR在这些基底前脑核中合成并运输到终末区域，在该区域神经生长因子被认为会与之结合并内化，这是这种神经营养因子众多作用的第一步。
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