Whittemore S R, Ebendal T, Lärkfors L, Olson L, Seiger A, Strömberg I, Persson H
Proc Natl Acad Sci U S A. 1986 Feb;83(3):817-21. doi: 10.1073/pnas.83.3.817.
The presence of nerve growth factor (NGF) mRNA and protein in the rat central nervous system is documented. Blot-hybridization analysis showed an abundance of NGF mRNA in the hippocampus, cerebral cortex, and olfactory bulb. Enzyme immunoassay confirmed significant levels of a NGF-like protein in the hippocampus and cerebral cortex. Bioassay of a NGF-like immunoaffinity-purified protein from these regions was physiologically indistinguishable from NGF. Immunohistochemistry revealed a widespread distribution of NGF-like reactivity in the adult brain, preferentially in fiber tracts. NGF mRNA accumulation began at birth, with adult levels reached 3 weeks postnatally. Enzyme immunoassay detected the presence of a NGF-like protein in the embryonic rat brain. Postnatally, the level of NGF-like protein reached a maximum at 3 weeks. Additionally, a distinct fetal form of NGF may exist.
已证实大鼠中枢神经系统中存在神经生长因子（NGF）mRNA和蛋白质。印迹杂交分析显示，海马体、大脑皮层和嗅球中存在大量NGF mRNA。酶免疫测定证实海马体和大脑皮层中存在显著水平的类NGF蛋白。对从这些区域经免疫亲和纯化的类NGF蛋白进行的生物测定在生理上与NGF无法区分。免疫组织化学显示，在成人大脑中，类NGF反应广泛分布，尤其在纤维束中。NGF mRNA在出生时开始积累，出生后3周达到成年水平。酶免疫测定在胚胎大鼠大脑中检测到类NGF蛋白的存在。出生后，类NGF蛋白水平在3周时达到最高。此外，可能存在一种独特的胎儿形式的NGF。
