白色念珠菌从酵母形态转变为菌丝形态过程中cAMP水平及cAMP相关酶的原位测量

cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans.

作者信息

Egidy G, Paveto C, Passeron S, Galvagno M A

机构信息

Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.

出版信息

Cell Biol Int Rep. 1990 Jan;14(1):59-68. doi: 10.1016/0309-1651(90)90071-6.

DOI:10.1016/0309-1651(90)90071-6

Abstract

Intracellular levels of cAMP and specific activities of adenylate cyclase, cAMP phosphodiesterase and cAMP-dependent protein kinase were measured during filamentation in the dimorphic fungus Candida albicans. Enzymatic assays were performed in permeabilized cells under conditions prevented endogenous proteolysis. The variations observed in cAMP levels were mainly accounted for by variations in the specific activities of adenylate cyclase and cAMP phosphodiesterase at different stages during germ tube formation. cAMP-dependent protein kinase, measured with kemptide as exogenous substrate, was developmental regulated. Some properties of the enzymatic activities from cell-free extracts are described.

摘要

在二态真菌白色念珠菌的丝状化过程中，测定了细胞内cAMP水平以及腺苷酸环化酶、cAMP磷酸二酯酶和cAMP依赖性蛋白激酶的比活性。酶活性测定在经通透处理的细胞中进行，条件是防止内源性蛋白水解。在芽管形成的不同阶段，cAMP水平的变化主要是由腺苷酸环化酶和cAMP磷酸二酯酶比活性的变化引起的。以kemptide作为外源底物测定的cAMP依赖性蛋白激酶受到发育调控。描述了无细胞提取物中酶活性的一些特性。

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