Kato K, Nakanishi M, Arata Y, Teshima R, Terao T, Miyamoto H, Hanaoka F, Ui M, Masuzawa Y, Hiraishi S
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Exp Cell Res. 1990 Jun;188(2):247-53. doi: 10.1016/0014-4827(90)90166-8.
We selected a new type of variant (designated 3C7) derived spontaneously from parental RBL-2H3 cells. 3C7 cells showed lower contact inhibition, anchorage dependency, and serotonin release activity than those of RBL-2H3 cells. We conclude that 3C7 cells are a transformant of RBL-2H3 cell with greater malignancy. The production of inositol bisphosphate and the release of Ca2+ from intracellular stores induced by IgE-antigen stimulation were enhanced in 3C7. Oscillation of [Ca2+]i in individual 3C7 cells was observed by a digital imaging microscopic technique. We propose that 3C7 cells are a useful model system for studies on the mechanisms of stimulus-secretion coupling and the relationships between malignant alterations and disorders of signal transduction.
我们从亲代RBL-2H3细胞中自发筛选出一种新型变体（命名为3C7）。与RBL-2H3细胞相比，3C7细胞表现出更低的接触抑制、贴壁依赖性和5-羟色胺释放活性。我们得出结论，3C7细胞是RBL-2H3细胞的一种具有更高恶性程度的转化体。在3C7中，由IgE-抗原刺激诱导的肌醇二磷酸的产生和细胞内储存的Ca2+释放增强。通过数字成像显微镜技术观察到单个3C7细胞中[Ca2+]i的振荡。我们认为3C7细胞是用于研究刺激-分泌偶联机制以及恶性改变与信号转导紊乱之间关系的有用模型系统。
