Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, São Paulo State University - UNESP, Araraquara, SP, Brazil.
J Adhes Dent. 2011 Apr;13(2):117-24. doi: 10.3290/j.jad.a19239.
To evaluate the effect of chlorhexidine on the presence of collagen in aged resin-dentin bonds produced on sound and caries-affected dentin.
Flat dentin surfaces were obtained from 16 sound molars, from which 8 were microbiologically processed for induction of caries. Single Bond 2 was applied to both sound and caries-affected substrates. In half of the teeth assigned for 6-month storage in water, the phosphoric acid demineralized dentin was impregnated with 2% chlorhexidine before the application of the adhesive. Specimens (2 x 2 x 5 mm) were produced and stored in water for 24 h, or 6 months in either water or mineral oil. The specimens were subjected to histological processing and sections were stained with Goldner's Trichrome. The thickness of the zone of exposed collagen was measured by optical microscopy and the data were subjected to two-way ANOVA and Tukey's test (α = 0.05).
There was no statistically significant difference (p > 0.05) between sound and caries-affected dentin regardless of the storage condition. For both substrates, significantly greater collagen exposure was observed after 6 months in water. Chlorhexidine-treated groups resulted in similar collagen exposure to that of the control and 6 months in water groups (p > 0.05), while no increase of the exposed collagen zone was observed after mineral oil storage.
Aging in water resulted in degradation of the resin-dentin bond, as demonstrated by the increase of the zone of exposed collagen. However, the degradation of the exposed collagen was decelerated in the presence of chlorhexidine.
评估洗必泰对在健康和龋损牙本质上形成的老化树脂-牙本质粘结中胶原存在的影响。
从 16 颗健康磨牙中获得平坦的牙本质表面，其中 8 颗经微生物处理以诱导龋损。将 Single Bond 2 应用于健康和龋损牙本质底物。在被分配用于在水中储存 6 个月的牙齿的一半中，在用粘结剂之前，用 2%洗必泰浸渍磷酸酸蚀牙本质。制作标本（2x2x5mm）并在水中储存 24 小时，或在水或矿物油中储存 6 个月。将标本进行组织学处理，并用 Goldner's Trichrome 染色切片。通过光学显微镜测量暴露胶原的区域的厚度，并将数据进行双因素方差分析和 Tukey 检验（α=0.05）。
无论储存条件如何，健康和龋损牙本质之间没有统计学上的显著差异（p>0.05）。对于两种底物，在水中储存 6 个月后，观察到明显更多的胶原暴露。洗必泰处理组的胶原暴露与对照组和水中储存 6 个月组相似（p>0.05），而在矿物油储存后未观察到暴露胶原区域的增加。
水老化导致树脂-牙本质粘结的降解，如暴露胶原区域的增加所示。然而，在洗必泰存在下，暴露胶原的降解被减缓。
