Araújo Eliane Avany Malveira, Lima Geisy Rebouças, Dos Santos de Melo Luciana Aleixo, de Sousa Leilane Bentes, de Vasconcellos Marne Carvalho, Conde Nikeila Chacon de Oliveira, Toda Carina, Hanan Simone Assayag, Alves Filho Ary de Oliveira, Bandeira Maria Fulgência Costa Lima
School of Dentistry, Amazonas Federal University,UFAM, Manaus, AM, Brazil.
School of Pharmaceutical Sciences, Amazonas Federal University,UFAM, Manaus, AM, Brazil.
Adv Pharmacol Pharm Sci. 2021 Feb 17;2021:8840570. doi: 10.1155/2021/8840570. eCollection 2021.
This study sets out to evaluate the antiproteolytic activity of copaiba oil-based emulsion at the resin/dentin adhesive interface union formed with conventional and self-etching adhesives systems.
At in situ zymography, 30 teeth were sectioned 2 mm below the enamel-dentin junction; a was standardized and subdivided into four groups. Gelatin conjugated with fluorescein was used and taken to the fluorescence microscope for evaluation. In cytotoxicity, the Trypan Blue method was used at four different time points. The tested groups were (G1) control with distilled water; (G2) 2% chlorhexidine (CLX); (G3) emulsion based on copaiba oil (EC) 10% + X; (G4) 10% EC + Y; and (G5) EC 10% alkaline. The zymographic assay used the same groups described, but in 30 seconds and 10 and 20 minutes. HT1080 cells were incubated and submitted to electrophoresis. The gel was analyzed using ImageJ software. Mann-Whitney and Kruskal-Wallis tests were used in the statistical analysis ( < 0.05).
ECs showed higher cell viability in the cytotoxicity test and showed a significant difference in 10 and 20 minutes. In the zymographic assay, alkaline EC reduced 67% of MMP-2 activity and 44% of MMP-9 compared to 2% chlorhexidine. At in situ zymography in qualitative evaluation, all groups tested showed inhibition of activity in metalloproteinases.
EC showed activity in the inhibition of metalloproteinases and in situ, especially the alkaline one. The survey shows the possibility of using ECs, a product from Amazonian biodiversity, as a biomodifier in dentistry.
本研究旨在评估基于巴西香脂油的乳剂在与传统和自酸蚀粘结系统形成的树脂/牙本质粘结界面结合处的抗蛋白水解活性。
在原位酶谱分析中，将30颗牙齿在釉质-牙本质交界处下方2毫米处切片；进行标准化并分为四组。使用与荧光素结合的明胶，并将其置于荧光显微镜下进行评估。在细胞毒性实验中，在四个不同时间点使用台盼蓝法。测试组包括（G1）蒸馏水对照组；（G2）2%氯己定（CLX）；（G3）10%+X的基于巴西香脂油的乳剂（EC）；（G4）10% EC+Y；以及（G5）10%碱性EC。酶谱分析使用上述相同分组，但在30秒、10分钟和20分钟时进行。将HT1080细胞进行孵育并进行电泳。使用ImageJ软件对凝胶进行分析。统计分析采用Mann-Whitney和Kruskal-Wallis检验（P<0.05）。
在细胞毒性测试中，ECs显示出较高的细胞活力，并且在10分钟和20分钟时显示出显著差异。在酶谱分析中，与2%氯己定相比，碱性EC使MMP-2活性降低了67%，MMP-9活性降低了44%。在原位酶谱分析的定性评估中，所有测试组均显示出金属蛋白酶活性受到抑制。
EC在抑制金属蛋白酶方面显示出活性，尤其是在原位，特别是碱性EC。该研究表明，作为一种来自亚马逊生物多样性的产品，ECs有作为牙科生物改性剂使用的可能性。
