Lenzi Tathiane Larissa, Calvo Ana Flávia Bissoto, Tedesco Tamara Kerber, Ricci Hérica Adad, Hebling Josimeri, Raggio Daniela Prócida
Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, Av. Prof. Lineu Prestes, 2227 - Cidade Universitária, São Paulo, SP, 05508-000, Brazil.
Department of Orthodontics and Pediatric Dentistry, Araraquara School of Dentistry, Universidade Estadual Paulista (Unesp), Rua Humaitá 1680, 14801-903, Araraquara, SP, Brazil.
BMC Oral Health. 2015 Jul 11;15:79. doi: 10.1186/s12903-015-0049-z.
To investigate the influence of chemical and microbiological methods of caries induction on bond degradation of adhesive systems to primary dentin.
Flat dentin surfaces from 36 primary molars were assigned to three groups (n = 12) according to method to induce caries-affected dentin: (1) control (sound dentin); (2) pH-cycling; and (3) microbiological caries induction model. Teeth were submitted to caries induction for 14 days for both methods, and the sound dentin was stored in distilled water during the same period. Specimens from each experimental group were then randomly reassigned to two subgroups (n = 6) according to the adhesive system tested: two-step etch-and-rinse adhesive (Adper Single Bond 2 - SB) or two-step self-etch system (Clearfil SE Bond - CSEB). Composite buildups were constructed and sectioned to obtain bonded sticks to be subjected to microtensile (μTBS) testing immediately or after 12 months of water aging. The μTBS means were analyzed by three-way repeated measures ANOVA and Tukey's tests (α = 0.05).
The μTBS values obtained to artificially-created caries-affected dentin were lower compared with sound dentin, but were not affected by method of caries induction. Water storage for 12 months reduced bond strengths, except to CSEB bonded to sound dentin.
Chemical and microbiological methods affect similarly the stability of resin-dentin bonds in primary teeth.
研究化学和微生物诱导龋齿的方法对粘结系统与乳牙牙本质粘结降解的影响。
根据诱导龋坏牙本质的方法,将36颗乳牙的平坦牙本质表面分为三组(每组n = 12):(1)对照组(健康牙本质);(2)pH循环组;(3)微生物诱导龋模型组。两种方法均对牙齿进行14天的龋诱导,在此期间健康牙本质保存在蒸馏水中。然后根据所测试的粘结系统,将每个实验组的标本随机重新分为两个亚组(每组n = 6):两步酸蚀冲洗粘结剂(Adper Single Bond 2 - SB)或两步自酸蚀系统(Clearfil SE Bond - CSEB)。构建复合树脂充填体并切片以获得粘结棒,立即或经过12个月水老化后进行微拉伸粘结强度(μTBS)测试。通过三因素重复测量方差分析和Tukey检验分析μTBS平均值(α = 0.05)。
与健康牙本质相比,人工诱导龋坏牙本质的μTBS值较低,但不受龋诱导方法的影响。除了CSEB粘结到健康牙本质外,12个月的水储存降低了粘结强度。
化学和微生物方法对乳牙中树脂 - 牙本质粘结稳定性的影响相似。