Department of Comparative Pathobiology, Purdue University, 406 South University Street, West Lafayette, IN 47907, USA.
Arch Virol. 2011 Sep;156(9):1525-35. doi: 10.1007/s00705-011-1020-1. Epub 2011 May 19.
Little is known about the neutralizing epitopes in turkey coronavirus (TCoV). The spike (S) protein gene of TCoV was divided into 10 fragments to identify the antigenic region containing neutralizing epitopes. The expression and antigenicity of S fragments was confirmed by immunofluorescence antibody (IFA) assay using an anti-histidine monoclonal antibody or anti-TCoV serum. Polyclonal antibodies raised against expressed S1 (amino acid position 1 to 573 from start codon of S protein), 4F/4R (482-678), 6F/6R (830-1071), or Mod4F/Epi4R (476-520) S fragment recognized native S1 protein and TCoV in the intestines of TCoV-infected turkey embryos. Anti-TCoV serum reacted with recombinant 4F/4R, 6F/6R, and Mod4F/Epi4R in a western blot. The results of a virus neutralization assay indicated that the carboxyl terminal region of the S1 protein (Mod4F/Epi4R) or the combined carboxyl terminal S1 and amino terminal S2 protein (4F/4R) possesses the neutralizing epitopes, while the S2 fragment (6F/6R) contains antigenic epitopes but not neutralizing epitopes.
关于火鸡冠状病毒（TCoV）的中和表位知之甚少。将 TCoV 的刺突（S）蛋白基因分成 10 个片段，以鉴定含有中和表位的抗原区域。通过使用抗组氨酸单克隆抗体或抗 TCoV 血清的免疫荧光抗体（IFA）测定来确认 S 片段的表达和抗原性。针对表达的 S1（从 S 蛋白起始密码子的 1 位到 573 位氨基酸）、4F/4R（482-678）、6F/6R（830-1071）或 Mod4F/Epi4R（476-520）S 片段产生的多克隆抗体识别天然 S1 蛋白和 TCoV 在 TCoV 感染的火鸡胚胎肠道中。抗 TCoV 血清在 Western blot 中与重组 4F/4R、6F/6R 和 Mod4F/Epi4R 反应。病毒中和测定的结果表明，S1 蛋白的羧基末端区域（Mod4F/Epi4R）或结合的羧基末端 S1 和氨基末端 S2 蛋白（4F/4R）具有中和表位，而 S2 片段（6F/6R）含有抗原表位但没有中和表位。
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