Department of Anatomy and Neurobiology, the Jiangsu Key Laboratory of Neuroregeneration, Nantong University, No. 19 Qixiu Road, Nantong, China.
In Vitro Cell Dev Biol Anim. 2011 Aug;47(7):431-7. doi: 10.1007/s11626-011-9422-7. Epub 2011 May 19.
The role of radial glia cells (RGCs) as neural progenitors and as guides for migrating neurons is well established, mouse or human-derived radial glia (RG)-like cells in vitro showed some astroglia and stem/progenitor properties like RGCs in vivo, but different species-derived RG-like cells present some different properties. Here we acquired rat-derived RG-like cells on adherent conditions in vitro and then identified their astroglia and stem/progenitor properties. Similarly to the RGCs, the RG-like cells could be double-labeled by brain lipid-binding protein, glial fibrillary acidic protein, vimentin with nestin and expressed some astroglia and stem/progenitor genes; these cells also presented tripotent differentiation potentialities, albeit the ability of gliogenesis far exceeded the neurogenesis in vitro. Taken together, we acquired and identified some properties of rat-derived RG-like cells from fetal cerebral cortices in vitro.
放射状胶质细胞 (RGCs) 作为神经前体细胞和迁移神经元的导向物的作用已得到充分证实，体外培养的源自小鼠或人类的放射状胶质样 (RG) 细胞具有一些类似于体内 RGCs 的星形胶质细胞和干细胞/祖细胞特性，但不同种属来源的 RG 样细胞具有一些不同的特性。在这里，我们在体外贴壁条件下获得了源自大鼠的 RG 样细胞，然后鉴定了它们的星形胶质细胞和干细胞/祖细胞特性。与 RGCs 类似，RG 样细胞可以被脑脂质结合蛋白、胶质纤维酸性蛋白、波形蛋白与巢蛋白双重标记，并表达一些星形胶质细胞和干细胞/祖细胞基因；这些细胞还具有三向分化潜能，尽管体外的神经发生能力远低于神经发生。总之，我们在体外从胎鼠大脑皮质中获得并鉴定了一些源自大鼠的 RG 样细胞的特性。
