Laboratory of Clinical Pharmacology and Toxicology, S. Maria delle Croci Ravenna Hospital, Viale Randi 5, Ravenna, IT 48100, Italy.
Rapid Commun Mass Spectrom. 2011 Jun 15;25(11):1552-8. doi: 10.1002/rcm.5029.
Recently, electrospray ionization mass spectroscopy (ESI-MS) has been widely used for the identification of drugs of abuse and their metabolites in biological samples. However, the sensitivity and selectivity of this technique are commonly inadequate for the analysis of tetrahydrocannabinol (THC) and its metabolites at very low levels, such as those sometimes required in forensic and clinical-legal applications. We coupled electrospray ionization and surface-activated chemical ionization (ESI-SACI) to various types of mass analyzers (ion trap, triple quadrupole and orbitrap) (ESI-SACI-MS) to improve the detection of 11-nor-9-carboxy-tetrahydrocannabinol (THC-COOH), the most common marker of THC abuse. The benefits of this approach in terms of sensitivity and selectivity compared with a common ESI-MS approach are clearly demonstrated.
最近，电喷雾电离质谱（ESI-MS）已广泛用于在生物样本中鉴定滥用药物及其代谢物。然而，这种技术的灵敏度和选择性通常不足以分析极低水平的四氢大麻酚（THC）及其代谢物，例如法医和临床法律应用中有时需要的水平。我们将电喷雾电离和表面活化化学电离（ESI-SACI）与各种类型的质量分析仪（离子阱、三重四极杆和轨道阱）（ESI-SACI-MS）相结合，以提高对 11-去甲-9-羧基四氢大麻酚（THC-COOH）的检测，这是最常见的 THC 滥用标志物。与常见的 ESI-MS 方法相比，该方法在灵敏度和选择性方面的优势得到了明显的证明。
