Departamento de Anatomía y Radiología, Facultad de Medicina, Universidad de Valladolid, Valladolid, Spain.
Dev Dyn. 2011 Jul;240(7):1650-9. doi: 10.1002/dvdy.22657. Epub 2011 May 18.
Embryonic-cerebrospinal fluid (E-CSF) plays crucial roles in early brain development including the control of neurogenesis. Although FGF2 and lipoproteins present in the E-CSF have previously been shown to be involved in neurogenesis, the main factor triggering this process remains unknown. E-CSF contains all-trans-retinol and retinol-binding protein involved in the synthesis of retinoic acid (RA), a neurogenesis inducer. In early chick embryo brain, only the mesencephalic-rombencephalic isthmus (IsO) is able to synthesize RA. Here we show that in chick embryo brain development: (1) E-CSF helps to control RA synthesis in the IsO by means of the RBP and all-trans-retinol it contains; (2) E-CSF has retinoic acid activity, which suggests it may act as a diffusion pathway for RA; and (3) the influence of E-CSF on embryonic brain neurogenesis is to a large extent due to its involvement in RA synthesis. These data help to understand neurogenesis from neural progenitor cells.
胚胎脑脊液（E-CSF）在早期大脑发育中发挥着关键作用，包括对神经发生的控制。尽管 E-CSF 中存在的 FGF2 和脂蛋白先前已被证明参与了神经发生，但触发这一过程的主要因素仍不清楚。E-CSF 中含有全反式视黄醇和视黄醇结合蛋白，参与了神经发生诱导剂视黄酸（RA）的合成。在早期鸡胚大脑中，只有中脑-后脑脑桥（IsO）能够合成 RA。在这里，我们表明在鸡胚大脑发育过程中：（1）E-CSF 通过其包含的 RBP 和全反式视黄醇有助于控制 IsO 中的 RA 合成；（2）E-CSF 具有视黄酸活性，这表明它可能作为 RA 的扩散途径；（3）E-CSF 对胚胎大脑神经发生的影响在很大程度上归因于其参与 RA 合成。这些数据有助于理解神经前体细胞的神经发生。
