Castells Anna, Parvas Maryam, Bueno David
Department of Genetics, University of Barcelona, Barcelona, Catalonia, Spain.
Neuroreport. 2012 Nov 14;23(16):917-21. doi: 10.1097/WNR.0b013e3283582067.
Embryonic cerebrospinal fluid (E-CSF) is a protein-containing fluid present in brain cavities that plays key roles in neuronal development and function. From the beginning of primary brain neurogenesis, E-CSF composition and homeostasis are precisely tuned by a transient blood-CSF barrier function, which controls protein transport and their relative concentration of within-brain cavities. One of the proteins found in E-CSF is ovalbumin, which is postulated to play a role in nutrition. Here, we address the question of whether neuroepithelial progenitor cells in developing chick embryos use ovalbumin as a highly specific nutritional source of amino acids or alternatively whether they use other amino acid sources, despite the fact that they cannot be transported from blood serum to brain cavities under physiological conditions. Although ovalbumin was not found to be a key protein required for neurogenesis and cell survival, our observations reinforce the crucial role of the embryonic blood-CSF barrier, as its precise regulation of protein transport and E-CSF homeostasis ensures the maximum efficiency of neural development.
胚胎脑脊液（E-CSF）是存在于脑腔中的一种含蛋白质的液体，在神经元发育和功能中起关键作用。从原发性脑神经元发生开始，E-CSF的组成和内环境稳定就通过短暂的血-脑脊液屏障功能进行精确调节，该功能控制蛋白质运输及其在脑腔内的相对浓度。在E-CSF中发现的一种蛋白质是卵清蛋白，据推测它在营养方面发挥作用。在这里，我们探讨了发育中的鸡胚神经上皮祖细胞是否将卵清蛋白用作高度特异性的氨基酸营养来源，或者尽管在生理条件下它们不能从血清转运到脑腔，它们是否使用其他氨基酸来源的问题。尽管未发现卵清蛋白是神经发生和细胞存活所需的关键蛋白质，但我们的观察结果强化了胚胎血-脑脊液屏障的关键作用，因为其对蛋白质运输和E-CSF内环境稳定的精确调节确保了神经发育的最大效率。
