Can Nafiz O, Arli Goksel, Lafci Yigit
Department of Analytical Chemistry, Faculty of Pharmacy, Anadolu University, Yunusemre Campus, Eskisehir, Turkey.
J Sep Sci. 2011 Aug;34(16-17):2214-22. doi: 10.1002/jssc.201100172. Epub 2011 May 19.
Potassium sorbate and sodium benzoate are food additives that are generally employed for prevention of food spoilage originating from bacteria, molds or yeasts. Although these compounds were generally recognized as safe due to their low risk of acute and chronic toxicity, they have limitations of usage to protect human health. Development and validation of a novel RP-HPLC method, in which a C18-bonded monolithic silica column was used as stationary phase to assay these compounds, is described for the first time. Aliquots of 10 μL of samples were injected into chromatograph and eluted using phosphate buffer (0.025 M, pH 2.0)-water-acetonitrile (50:45:5, v/v/v) solution, which was pumped at the rate of 3.0 mL/min. To sharpen the peaks, 10 mM octylamine was added to the mobile phase. Potassium sorbate and sodium benzoate were detected at about 12(th) and 14(th) min, respectively, and quantified at 230 nm using photodiode array detector. A total of 41 samples were prepared by simply filtering through 0.45 μm filters after sonication, and injected into the system without any pre-treatment steps. Applicability of the method was demonstrated by performing total procedure on samples of different brands and types, and their compliance to official regulations was assessed.
山梨酸钾和苯甲酸钠是食品添加剂，通常用于防止由细菌、霉菌或酵母菌引起的食品变质。尽管这些化合物因其急性和慢性毒性风险较低而被普遍认为是安全的，但它们在保护人类健康方面存在使用限制。首次描述了一种新型反相高效液相色谱法（RP-HPLC）的开发和验证，该方法使用C18键合整体硅胶柱作为固定相来测定这些化合物。将10μL样品等分试样注入色谱仪，并用磷酸盐缓冲液（0.025M，pH 2.0）-水-乙腈（50:45:5，v/v/v）溶液洗脱，该溶液以3.0mL/min的流速泵送。为了使峰变尖锐，向流动相中加入10mM辛胺。山梨酸钾和苯甲酸钠分别在约12分钟和14分钟时被检测到，并使用光电二极管阵列检测器在230nm处进行定量。通过在超声处理后简单地通过0.45μm滤膜过滤制备了总共41个样品，并且在没有任何预处理步骤的情况下将其注入系统。通过对不同品牌和类型的样品进行整个程序来证明该方法的适用性，并评估它们是否符合官方规定。
