a Food and Drug Administration , Tehran University of Medical Sciences , Tehran , Iran.
Food Addit Contam Part B Surveill. 2014;7(2):115-9. doi: 10.1080/19393210.2013.857726. Epub 2014 Jan 16.
Sodium benzoate and potassium sorbate are two common preservatives used in Iran, yet use of these preservatives in doogh (Iranian dairy-based drink) is forbidden according to national standards. The aim of this study was to consider the presence of these preservatives in doogh by high-performance liquid chromatography with UV detection (HPLC-UV). The method was performed using a C18 column and detection at 225 nm. The mobile phase contained ammonium acetate buffer (pH = 4.2) and acetonitrile (80:20 v/v). The survey included 130 samples of doogh for identification and quantification of the named preservatives. All samples contained sodium benzoate, but potassium sorbate was detected in only 13% of them. The means of benzoate and sorbate were 21.3 ± 2.7 and 13.3 ± 39.6 mg kg(-1), respectively. The limits of detection were 2 and 40 ng g(-1) for benzoate and sorbate, respectively. Results indicate that sodium benzoate may occur in doogh naturally.
苯甲酸钠和山梨酸钾是伊朗常用的两种防腐剂，但根据国家标准，这些防腐剂禁止用于驼奶酒（伊朗的一种乳基饮料）。本研究旨在采用高效液相色谱法结合紫外检测（HPLC-UV）来检测驼奶酒中是否含有这些防腐剂。该方法使用 C18 柱，在 225nm 处进行检测。流动相含有乙酸铵缓冲液（pH=4.2）和乙腈（80:20v/v）。该调查包括 130 份驼奶酒样品，用于鉴定和定量检测这两种防腐剂。所有样品均含有苯甲酸钠，但只有 13%的样品中检测到山梨酸钾。苯甲酸钠和山梨酸钾的平均值分别为 21.3±2.7mg/kg 和 13.3±39.6mg/kg。苯甲酸钠和山梨酸钾的检出限分别为 2ng/g 和 40ng/g。结果表明，苯甲酸钠可能会自然存在于驼奶酒中。
