de Sousa Ana Paula Cavalcanti, de Aguiar Valença Neto Alberto de Aguiar Pires, Marchionni Antônio Márcio Teixeira, de Araújo Ramos Marília, dos Reis Júnior João Alves, Pereira Manoela Carrera Martinez Cavalcante, Cangussú Maria Cristina Teixeira, de Almeida Reis Silvia Regina, Pinheiro Antônio Luiz Barbosa
Center of Biophotonics, School of Dentistry, Federal University of Bahia, Salvador, Bahia, Brazil.
Photomed Laser Surg. 2011 Sep;29(9):605-11. doi: 10.1089/pho.2010.2833. Epub 2011 May 19.
The aim of the present investigation was to evaluate transforming growth factor β (TGF-β) expression on cutaneous wounds in rodents treated or not treated with LED light.
TGF-β is a multifunctional cytokine that presents a central action during tissue repair. Although several studies both in vitro and in vivo have shown that LED phototherapy influences tissue repair, a full understanding of the mechanisms involved in its usage, such as in the modulation of some growth factors, remains unclear.
Under general anesthesia, 24 young adult male Wistar rats weighing 200-250 g had one excisional wound created on the dorsum of each, and were randomly distributed into two groups: G0 (Control) and G1 (LED, λ700 ± 20 nm, 16 mW, SAEF = 5 J/cm(2), Illuminated Area = 2 cm(2), 8 mWcm(2), 626 s) Each group was subdivided into three subgroups according to the animal death timing (2, 4, and 6 days). LED phototherapy started immediately after surgery and was repeated every other day during the experimental time. Following animal death, specimens were removed, routinely processed to wax, cut and immunomarked with polyclonal anti-TGF-β, and underwent histological analysis by light microscopy. The mean area of expression of each group was calculated. The data were statistically analyzed using ANOVA and Tukey's test.
The area of the expression of TGF-β on LED-irradiated animals was significantly smaller than on controls at day 2 (p = 0.013). No significant difference was found at later times. It is concluded that the use of LED light, at these specific parameters, caused an inhibition of the expression of TGF-β at an early stage of the healing process.
本研究旨在评估经LED光处理或未处理的啮齿动物皮肤伤口上转化生长因子β（TGF-β）的表达情况。
TGF-β是一种多功能细胞因子，在组织修复过程中发挥核心作用。尽管多项体外和体内研究表明LED光疗会影响组织修复，但对于其作用机制，如对某些生长因子的调节，仍不完全清楚。
在全身麻醉下，24只体重200 - 250克的年轻成年雄性Wistar大鼠，每只在背部制造一个切除伤口，并随机分为两组：G0（对照组）和G1（LED组，波长700 ± 20纳米，16毫瓦，SAEF = 5焦耳/平方厘米，照射面积 = 2平方厘米，8毫瓦/平方厘米，626秒）。根据动物死亡时间（2天、4天和6天），每组再分为三个亚组。LED光疗在手术后立即开始，并在实验期间每隔一天重复一次。动物死亡后，取出标本，常规处理成蜡块，切片并用抗TGF-β多克隆抗体进行免疫标记，然后通过光学显微镜进行组织学分析。计算每组的平均表达面积。数据采用方差分析和Tukey检验进行统计学分析。
在第2天，接受LED照射的动物中TGF-β的表达面积显著小于对照组（p = 0.013）。在随后的时间里未发现显著差异。结论是，在这些特定参数下使用LED光会在愈合过程的早期抑制TGF-β的表达。
