Department of Pharmacology, School of Preclinical and Forensic Medicine, West China Medical Center, Sichuan University, Chengdu, P.R. China.
Pharm Biol. 2011 Oct;49(10):1058-64. doi: 10.3109/13880209.2011.565059. Epub 2011 May 20.
The traditional herbal medicinal formula Da-Cheng-Qi decoction (DCQD) has long been used to treat pancreatitis in China; however, the underlying mechanisms remain unclear.
To investigate whether DCQD is beneficial to the patients with lung injury in severe acute pancreatitis (SAP); if it is, then to explore the lung protective effect of DCQD and the mechanism involved in rats.
DCQD was enema administered to 70 patients for 7 days. Mortality, (multi)organ failure during admission were observed, blood samples for laboratory analysis were drawn on admission, on Days 3, 7, and 14 of the treatment. We also experimentally examined the function of two doses of DCQD in SAP rat models. IL-1β, IL-6, and IL-10 mRNA expression in rat lungs was measured quantitatively by the RT-PCR method and confirmed by morphometric studies of the lungs.
It was demonstrated that the administration of DCQD did shorten the average time that patients suffered acute respiratory distress syndrome (ARDS). Compared with untreated rats, the lungs of rats treated with DCQD showed significantly lower levels of proinflammatory cytokine IL-1β and IL-6 mRNA. Rats treated with DCQD had lower mean pathological lung lesion scores than those in SAP rats.
DCQD has good prospects in the treatment for SAP because it did shorten the average time that patients suffered ARDS in the clinic. It exerts therapeutic effects on this disease through inhibiting the production of inflammatory mediators, decreasing the anti-inflammatory factors, and mitigating the pathological damage of the lung injury in SAP model.
大承气汤（DCQD）作为一种传统的中草药配方，长期以来一直被用于治疗中国的胰腺炎；然而，其潜在机制尚不清楚。
研究 DCQD 是否有益于重症急性胰腺炎（SAP）患者的肺损伤；如果是，那么探索 DCQD 在大鼠中的肺保护作用及其涉及的机制。
对 70 例患者进行了 7 天的 DCQD 灌肠治疗。观察住院期间的死亡率和（多）器官衰竭，入院时、治疗第 3、7 和 14 天采集血液样本进行实验室分析。我们还在 SAP 大鼠模型中实验研究了两种剂量的 DCQD 的功能。通过 RT-PCR 方法定量测量大鼠肺中 IL-1β、IL-6 和 IL-10 mRNA 的表达，并通过对肺的形态计量学研究进行验证。
结果表明，DCQD 的给药可缩短患者急性呼吸窘迫综合征（ARDS）的平均发病时间。与未治疗的大鼠相比，用 DCQD 治疗的大鼠的肺部促炎细胞因子 IL-1β 和 IL-6 mRNA 水平显著降低。用 DCQD 治疗的大鼠的平均病理肺损伤评分低于 SAP 大鼠。
DCQD 在 SAP 的治疗中有很好的应用前景，因为它确实缩短了患者患 ARDS 的平均时间。它通过抑制炎症介质的产生、减少抗炎因子和减轻 SAP 模型中肺损伤的病理损伤，对这种疾病发挥治疗作用。
