Centro de Biotecnologia, Instituto de Pesquisas Energéticas e Nucleares, Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, São Paulo, Brazil.
Artif Organs. 2011 May;35(5):502-7. doi: 10.1111/j.1525-1594.2011.01255.x.
Grafts of biological tissues have been used since the 1960s as an alternative to the mechanical heart prostheses. Nowadays, the most consolidated treatment to bovine pericardial (BP) bioprostheses is the crosslinking with glutaraldehyde (GA), although GA may induce calcification in vivo. In previous work, our group demonstrated that electron beam irradiation applied to lyophilized BP in the absence of oxygen promoted crosslinks among collagen fibers of BP tissue. In this work, the incorporation of silk fibroin (SF) and chitosan (CHIT) in the BP not treated with GA was studied. The samples were irradiated and then analyzed for their cytotoxicity and the ability of adhesion and growth of endothelial cells. Initially, all samples showed cytotoxicity. However, after a few washing cycles, the cytotoxicity due to acetic acid and ethanol residues was removed from the biomaterial making it suitable for the biofunctional test. The samples modified with SF/CHIT and electron beam irradiated favored the adhesion and growth of endothelial cells throughout the tissue.
自 20 世纪 60 年代以来，生物组织移植物已被用作机械心脏假体的替代品。如今，牛心包 (BP) 生物假体最成熟的处理方法是用戊二醛 (GA) 交联，尽管 GA 可能会在体内诱导钙化。在之前的工作中，我们小组证明了在没有氧气的情况下对冻干 BP 应用电子束辐照会促进 BP 组织胶原纤维之间的交联。在这项工作中，研究了丝素蛋白 (SF) 和壳聚糖 (CHIT) 在未经 GA 处理的 BP 中的掺入。对样品进行辐照，然后分析其细胞毒性以及内皮细胞黏附和生长的能力。最初，所有样品都显示出细胞毒性。然而，经过几次洗涤循环后，生物材料中去除了由于残留的乙酸和乙醇引起的细胞毒性，使其适合进行生物功能测试。经 SF/CHIT 修饰并经电子束辐照的样品有利于内皮细胞在整个组织中的黏附和生长。
