Department of Food & Nutrition, Education Graduate School, Kyung Hee University, Hoegi-dong, Dongdaemun-gu, Seoul, Republic of Korea.
Eur J Pharmacol. 2011 Aug 16;664(1-3):1-7. doi: 10.1016/j.ejphar.2011.05.005. Epub 2011 May 11.
The dried root of Saussurea lappa Clarke (Compositae) has been used as a traditional medicine. Dehydrocostus lactone is one of the main bioactive constituents of this medicinal plant. In the present study, the protective effect of dehydrocostus lactone against antimycin A (an inhibitor of mitochondrial complex III)-induced cytotoxicity was investigated in osteoblastic MC3T3-E1 cells. Pre-treatment with dehydrocostus lactone prior to antimycin A exposure significantly prevented mitochondrial membrane potential dissipation, complex IV inactivation, ATP loss, cytochrome c release, intracellular calcium elevation and potassium loss, and reactive oxygen species production induced by antimycin A. These results suggest that dehydrocostus lactone protects osteoblastic MC3T3-E1 cells from antimycin A-induced cell damage through the improved mitochondrial function.
积雪草（伞形科）的干根被用作传统药物。去氢木香内酯是这种药用植物的主要生物活性成分之一。在本研究中，研究了去氢木香内酯对抗霉素 A（一种线粒体复合物 III 抑制剂）诱导的细胞毒性的保护作用在成骨细胞 MC3T3-E1 细胞中。在暴露于抗霉素 A 之前用去氢木香内酯预处理可显著防止线粒体膜电位耗散、复合物 IV 失活、ATP 损失、细胞色素 c 释放、细胞内钙升高和钾丢失以及抗霉素 A 诱导的活性氧产生。这些结果表明，去氢木香内酯通过改善线粒体功能来保护成骨细胞 MC3T3-E1 细胞免受抗霉素 A 诱导的细胞损伤。
