Research Institute of Endocrinology, Kyung Hee University Hospital, 1, Hoegi-dong, Dongdaemun-gu, Seoul 130-702, South Korea.
Biol Trace Elem Res. 2013 Jun;153(1-3):428-36. doi: 10.1007/s12011-013-9679-7. Epub 2013 May 5.
Gold nanoparticles have shown promising biological applications due to their unique properties. Understanding the interaction mechanisms between nanomaterials and biological cells is important for the control and manipulation of these interactions for biomedical applications. In the present study, we investigated the effects of gold nanoparticles on the differentiation of osteoblastic MC3T3-E1 cells and antimycin A-induced mitochondrial dysfunction. The results showed that gold nanoparticles (5, 10, and 20 nm) caused a significant elevation of cell growth, alkaline phosphatase activity, collagen synthesis, and osteocalcin content in the cells (P < 0.05). Moreover, pretreatment with gold nanoparticles prior to antimycin A exposure significantly reduced antimycin A-induced cell damage by preventing mitochondrial membrane potential dissipation, complex IV inactivation, ATP loss, cytochrome c release, cardiolipin peroxidation, and reactive oxygen species generation. Taken together, our study indicated that gold nanoparticles may improve the differentiation and have protective effects on mitochondrial dysfunction of osteoblastic cells.
金纳米颗粒由于其独特的性质，在生物医学领域具有广阔的应用前景。了解纳米材料与生物细胞之间的相互作用机制，对于控制和操纵这些相互作用以应用于生物医学领域非常重要。在本研究中，我们研究了金纳米颗粒对成骨细胞 MC3T3-E1 细胞分化和抗霉素 A 诱导的线粒体功能障碍的影响。结果表明，金纳米颗粒（5、10 和 20nm）显著提高了细胞的生长、碱性磷酸酶活性、胶原合成和骨钙素含量（P<0.05）。此外，在暴露于抗霉素 A 之前用金纳米颗粒预处理，通过防止线粒体膜电位耗散、复合物 IV 失活、ATP 损失、细胞色素 c 释放、心磷脂过氧化和活性氧生成，显著减轻了抗霉素 A 诱导的细胞损伤。综上所述，我们的研究表明，金纳米颗粒可能促进成骨细胞的分化，并对其线粒体功能障碍具有保护作用。
