Department of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands.
FEBS Lett. 2011 Jun 23;585(12):1711-6. doi: 10.1016/j.febslet.2011.05.017. Epub 2011 May 13.
Platelets tightly regulate haemostasis and arterial thrombosis. Protein kinase C (PKC) is involved in most platelet responses implicated in thrombus formation. Recent pharmacological and mouse gene knockout approaches show that the conventional PKC isoforms and the novel PKC isoforms contribute in distinct ways to these platelet responses. We hypothesize that, in platelets and other cells, the characteristic functions of PKC isoforms are established through unique activation mechanisms and unique interacting protein partners, which result in isoform-specific patterns of substrate phosphorylation. For identifying the substrate proteins in a living cell, new methodology is available and discussed.
血小板可紧密调节止血和动脉血栓形成。蛋白激酶 C（PKC）参与大多数与血栓形成相关的血小板反应。最近的药理学和小鼠基因敲除方法表明，传统 PKC 同工型和新型 PKC 同工型以不同的方式对这些血小板反应做出贡献。我们假设，在血小板和其他细胞中，PKC 同工型的特征功能是通过独特的激活机制和独特的相互作用蛋白伴侣建立的，从而导致底物磷酸化的同工型特异性模式。为了鉴定活细胞中的底物蛋白，新的方法学已经出现并进行了讨论。
