Animal Influenza Laboratory of Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
Vet Microbiol. 2011 Sep 28;152(3-4):229-34. doi: 10.1016/j.vetmic.2011.04.032. Epub 2011 Apr 30.
Influenza A (H1N1) virus has caused human influenza outbreaks in a worldwide pandemic since April 2009. Pigs have been found to be susceptible to this influenza virus under experimental and natural conditions, raising concern about their potential role in the pandemic spread of the virus. In this study, we generated a high-growth reassortant virus (SC/PR8) that contains the hemagglutinin (HA) and neuraminidase (NA) genes from a novel H1N1 isolate, A/Sichuan/1/2009 (SC/09), and six internal genes from A/Puerto Rico/8/34 (PR8) virus, by genetic reassortment. The immunogenicity and protective efficacy of this reassortant virus were evaluated at different doses in a challenge model using a homologous SC/09 or heterologous A/Swine/Guangdong/1/06(H1N2) virus (GD/06). Two doses of SC/PR8 virus vaccine elicited high-titer serum hemagglutination inhibiting (HI) antibodies specific for the 2009 H1N1 virus and conferred complete protection against challenge with either SC/09 or GD/06 virus, with reduced lung lesions and viral shedding in vaccine-inoculated animals compared with non-vaccinated control animals. These results indicated for the first time that a high-growth SC/PR8 reassortant H1N1 virus exhibits properties that are desirable to be a promising vaccine candidate for use in swine in the event of a pandemic H1N1 influenza.
自 2009 年 4 月以来，甲型 H1N1 流感病毒已在全球范围内引发了人类流感大流行。实验和自然条件下发现猪易感染这种流感病毒，这引起了人们对其在病毒大流行传播中潜在作用的关注。在这项研究中，我们通过遗传重配产生了一种高生长重组病毒（SC/PR8），该病毒含有来自新型 H1N1 分离株 A/Sichuan/1/2009（SC/09）的血凝素（HA）和神经氨酸酶（NA）基因，以及来自 A/Puerto Rico/8/34（PR8）病毒的六个内部基因。我们在同源性 SC/09 或异源性 A/Swine/Guangdong/1/06（H1N2）病毒（GD/06）的攻毒模型中，评估了该重组病毒在不同剂量下的免疫原性和保护效力。两剂 SC/PR8 病毒疫苗可诱导针对 2009 年 H1N1 病毒的高滴度血清血凝抑制（HI）抗体，并可完全保护免受 SC/09 或 GD/06 病毒的攻击，与未接种疫苗的对照动物相比，接种疫苗的动物的肺部病变和病毒脱落减少。这些结果首次表明，高生长性 SC/PR8 重组 H1N1 病毒具有理想的特性，可成为大流行 H1N1 流感发生时用于猪的有前途的疫苗候选物。
