Graduate School of Energy and Environment, Seoul National University of Science and Technology, 172 Gongneung-2 Dong, Nowon-Gu, Seoul 139-743, South Korea.
Waste Manag. 2011 Sep-Oct;31(9-10):2121-5. doi: 10.1016/j.wasman.2011.04.011. Epub 2011 May 18.
Food waste generated in Korea is rich in carbohydrate as high as 65% of total solids. Using the food waste, the feasibility of ethanol production was investigated in a lab-scale fermentor. Pretreatment with hydrolyzing enzymes including carbohydrase, glucoamylase, cellulase and protease were tested for hydrolysis of food waste. The carbohydrase was able to hydrolyze and produce glucose with a glucose yield of 0.63 g glucose/g total solid. Enzymatic hydrolysis and ethanol fermentation by using carbohydrase and Saccharomyces cerevisiae were conducted in the batch mode. For separated hydrolysis and fermentation (SHF), ethanol concentration reached at the level corresponding to an ethanol yield of 0.43 g ethanol/g total solids. For simultaneous saccharification and fermentation (SSF), the ethanol yield was 0.31 g ethanol/g total solids. During the continuous operation of SHF, the volumetric ethanol production rate was 1.18 g/lh with an ethanol yield of 0.3g ethanol/g total solids. For SSF process, the volumetric ethanol production rate was 0.8 g/lh with an ethanol yield of 0.2g ethanol/g total solids.
韩国产生的食物垃圾富含碳水化合物,高达总固体的 65%。在实验室规模的发酵罐中,利用食物垃圾研究了生产乙醇的可行性。测试了包括碳水化合物酶、糖化酶、纤维素酶和蛋白酶在内的水解酶对食物垃圾的水解作用。碳水化合物酶能够水解并产生葡萄糖,葡萄糖得率为 0.63 g 葡萄糖/总固体。在分批模式下,使用碳水化合物酶和酿酒酵母进行了酶解和乙醇发酵。对于分相水解和发酵(SHF),乙醇浓度达到了对应于 0.43 g 乙醇/总固体的乙醇产率的水平。对于同步糖化和发酵(SSF),乙醇产率为 0.31 g 乙醇/总固体。在 SHF 的连续操作过程中,体积乙醇生产速率为 1.18 g/lh,乙醇产率为 0.3 g 乙醇/总固体。对于 SSF 工艺,体积乙醇生产速率为 0.8 g/lh,乙醇产率为 0.2 g 乙醇/总固体。