Zhu Mingjun, Li Ping, Gong Xinfang, Wang Jufang
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou Higher Education Mega Center, Panyu, Guangzhou, People's Republic of China.
Biosci Biotechnol Biochem. 2012;76(4):671-8. doi: 10.1271/bbb.110750. Epub 2012 Apr 7.
The processes of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) were employed using Saccharomyces cerevisiae for the production of ethanol from cassava pulp without any pretreatment. A combination of amylase, cellulase, cellobiase, and glucoamylase produced the highest levels of ethanol production in both the SHF and the SSF method. A temperature of 37 °C, a pH of 5.0, and an inoculum size of 6% were the optimum conditions for SSF. For the batch process at a pulp concentration of 20%, ethanol production levels from SHF and SSF were the highest, at 23.51 and 34.67 g L(-1) respectively, but in the fed-batch process, the levels of ethanol production from SHF and SSF rose to 29.39 and 43.25 g L(-1) respectively, which were 25% and 24.7% higher than those of the batch process. Thus SSF using the fed-batch provided a more efficient method for the utilization of cassava pulp.
采用酿酒酵母，在不进行任何预处理的情况下，利用木薯渣通过单独水解发酵（SHF）和同步糖化发酵（SSF）工艺生产乙醇。淀粉酶、纤维素酶、纤维二糖酶和糖化酶的组合在SHF和SSF方法中均产生了最高水平的乙醇产量。37℃的温度、5.0的pH值和6%的接种量是SSF的最佳条件。对于纸浆浓度为20%的分批过程，SHF和SSF的乙醇产量最高，分别为23.51和34.67 g L(-1)，但在补料分批过程中，SHF和SSF的乙醇产量分别升至29.39和43.25 g L(-1)，比分批过程分别高出25%和24.7%。因此，补料分批的SSF为木薯渣的利用提供了一种更有效的方法。
