RNAP Laboratory, Institute for Structural and Molecular Biology, Division of Biosciences, University College London, Darwin Building, Gower Street, London WC1E 6BT, United Kingdom.
Curr Opin Microbiol. 2011 Jun;14(3):328-34. doi: 10.1016/j.mib.2011.04.012. Epub 2011 May 17.
RNA polymerases (RNAPs) make repeatedly use of their templates by cycling through initiation, elongation and termination phases of transcription; during each step RNAP is interacting with and regulated by distinct transcription factors. The dynamic interplay between nucleic acid sequences, transcription factors and RNAP affects the activity and distribution of transcription complexes across the genome, and ultimately executes the genetic programme of the organism. This review covers recent discoveries about the mechanisms of archaeal transcription obtained by a combination of in vivo and in vitro approaches, from the molecular to the global level.
RNA 聚合酶 (RNAPs) 通过转录的起始、延伸和终止阶段循环使用其模板；在每个步骤中，RNAP 与不同的转录因子相互作用并受其调控。核酸序列、转录因子和 RNAP 之间的动态相互作用影响转录复合物在基因组上的活性和分布，并最终执行生物体的遗传程序。这篇综述涵盖了通过体内和体外方法相结合从分子到全局水平获得的关于古菌转录机制的最新发现。
