Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA.
Mol Plant. 2011 Jul;4(4):748-58. doi: 10.1093/mp/ssr036. Epub 2011 May 18.
Chromatin is the primary carrier of epigenetic information in higher eukaryotes. AtCYP71 contains both cyclophilin domain and WD40 repeats. Loss of AtCYP71 function causes drastic pleiotropic phenotypic defects. Here, we show that AtCYP71 physically interacts with FAS1 and LHP1, respectively, to modulate their distribution on chromatin. The lhp1 cyp71 double mutant showed more severe phenotypes than the single mutants, suggesting that AtCYP71 and LHP1 synergistically control plant development. Such synergism was in part illustrated by the observation that LHP1 association with its specific target loci requires AtCYP71 function. We also demonstrate that AtCYP71 physically interacts with FAS1 and is indispensable for FAS1 targeting to the KNAT1 locus. Together, our data suggest that AtCYP71 is involved in fundamental processes of chromatin assembly and histone modification in plants.
染色质是高等真核生物中表观遗传信息的主要载体。AtCYP71 含有亲环素结构域和 WD40 重复序列。AtCYP71 功能的丧失会导致严重的表型多效缺陷。在这里，我们表明 AtCYP71 分别与 FAS1 和 LHP1 相互作用，以调节它们在染色质上的分布。lhp1 cyp71 双突变体比单突变体表现出更严重的表型，这表明 AtCYP71 和 LHP1 协同控制植物发育。这种协同作用部分体现在观察到 LHP1 与其特定靶标基因座的结合需要 AtCYP71 功能。我们还证明 AtCYP71 与 FAS1 相互作用，并且对于 FAS1 靶向 KNAT1 基因座是不可或缺的。总之，我们的数据表明 AtCYP71 参与了植物染色质组装和组蛋白修饰的基本过程。
