Department of Molecular Virology, Cyprus Institute of Neurology and Genetics, PO Box 23462, 1683 Nicosia, Cyprus.
Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki, Greece.
J Med Microbiol. 2011 Oct;60(Pt 10):1433-1440. doi: 10.1099/jmm.0.029892-0. Epub 2011 May 19.
Human enteroviruses (HEVs) are responsible for a wide spectrum of clinical diseases. Even though usually associated with non-specific febrile illness, they are the most common cause of viral meningitis and pose a serious public-health problem, especially during outbreaks. Rapid detection and identification of HEV serotypes in clinical specimens are important in appropriate patient management and epidemiological investigation. A 5 year study (2003-2007) of clinical specimens from patients with viral meningitis and/or symptoms of enteroviral infection was carried out in Cyprus to determine the underlying enteroviral aetiology. Reverse transcription, followed by a sequential PCR strategy targeting the 5' non-coding region and VP1 region, was used for typing the isolated enteroviruses. The serotype of each isolate was determined by blast search of the VP1 amplicon sequence against GenBank. Clinical specimens from a total of 146 patients were diagnosed as enterovirus-positive. Twenty-two different serotypes were identified. The main strains identified were echovirus 18 and echovirus 30, followed by coxsackievirus B5, echovirus 9, echovirus 6, coxsackievirus A10 and coxsackievirus B2. However, rapid changes in serotype frequency and diversity were observed over time. Serotype distribution corresponded essentially with observations reported from other European countries in the same period. The present report demonstrates the epidemiology of enteroviruses in Cyprus from 2003 to 2007.
人类肠道病毒（HEVs）可引起广泛的临床疾病。虽然它们通常与非特异性发热性疾病相关，但却是病毒性脑膜炎的最常见病因，构成严重的公共卫生问题，尤其是在暴发期间。在临床标本中快速检测和鉴定 HEV 血清型对于适当的患者管理和流行病学调查非常重要。在塞浦路斯进行了一项为期 5 年的（2003-2007 年）研究，以确定病毒性脑膜炎和/或肠道病毒感染症状患者的潜在肠道病毒病因。采用逆转录和随后针对 5'非编码区和 VP1 区的连续 PCR 策略对分离的肠道病毒进行分型。通过对 VP1 扩增子序列与 GenBank 的 Blast 搜索确定每个分离株的血清型。总共从 146 例患者的临床标本中诊断为肠道病毒阳性。鉴定出 22 种不同的血清型。主要鉴定的菌株是埃可病毒 18 和埃可病毒 30，其次是柯萨奇病毒 B5、埃可病毒 9、埃可病毒 6、柯萨奇病毒 A10 和柯萨奇病毒 B2。然而，随着时间的推移，血清型频率和多样性迅速变化。血清型分布与同期其他欧洲国家的观察结果基本一致。本报告展示了 2003 年至 2007 年塞浦路斯的肠道病毒流行病学情况。
