National Centre for Microbiology, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain.
J Clin Virol. 2010 Feb;47(2):170-6. doi: 10.1016/j.jcv.2009.11.013. Epub 2009 Dec 16.
Human enteroviruses (HEV) are the commonest cause of viral meningitis as well as other pathologies, therefore HEV characterization is important both in patient management and epidemiological investigation.
A 10-year study of patients with enteroviral infection was carried out in Spain to determine the underlying etiology.
HEV were fully typed by microneutralisation tests and/or molecular methods.
A collection of 86404 clinical samples were studied in several Spanish laboratories. These were collected from patients with different syndromes, mainly aseptic meningitis (AM), fever, respiratory diseases and acute flaccid paralysis. Of these, 6867 HEV were obtained. At the National Poliovirus Laboratory 2814 were serotypically characterised. Among non-polio enteroviruses, the eight main serotypes were Echovirus 30 (25%), Echovirus 6 (12.4%), Echovirus 13 (8.3%), Echovirus 11 (7.4%) and Echovirus 9 (4.7%), followed by Coxsackievirus B5 (4.2%) and Echovirus 7 and Coxsackievirus A9 (3.7%) each. In AM cases, Echovirus 30 was identified in 39% of them, followed by Echovirus 6 (14%). However, Echovirus 6 was mainly associated with respiratory disease (17%), followed by Echovirus 11 (10%). On the other hand, Echovirus 30, Echovirus 11 and Echovirus 6 contributed equally with 12% of each serotype in the cases of fever.
The present report complements previous data (Trallero et al.(13)), with the results of HEV incidence in Spain from 1998 to 2007. The surveillance described in this study provided valuable information as to which serotypes are in circulation, the emergence of new HEV and association with clinical manifestations.
人类肠道病毒（HEV）是病毒性脑膜炎以及其他疾病的最常见病因，因此 HEV 的特征分析对于患者管理和流行病学研究都非常重要。
在西班牙进行了一项为期 10 年的肠病毒感染患者研究，以确定潜在病因。
通过微量中和试验和/或分子方法对 HEV 进行了全面分型。
在西班牙的几个实验室中研究了 86404 份临床样本。这些样本来自具有不同综合征的患者，主要是无菌性脑膜炎（AM）、发热、呼吸道疾病和急性弛缓性麻痹。其中，获得了 6867 株 HEV。在国家脊灰病毒实验室，对 2814 株进行了血清型特征分析。在非脊灰肠道病毒中，八个主要血清型是埃可病毒 30（25%）、埃可病毒 6（12.4%）、埃可病毒 13（8.3%）、埃可病毒 11（7.4%）和埃可病毒 9（4.7%），其次是柯萨奇病毒 B5（4.2%）和埃可病毒 7 和柯萨奇病毒 A9（3.7%）。在 AM 病例中，39%的病例中发现了埃可病毒 30，其次是埃可病毒 6（14%）。然而，埃可病毒 6 主要与呼吸道疾病相关（17%），其次是埃可病毒 11（10%）。另一方面，埃可病毒 30、埃可病毒 11 和埃可病毒 6 在发热病例中各占 12%，其血清型比例相同。
本报告补充了之前的报告（Trallero 等人（13）），提供了 1998 年至 2007 年西班牙 HEV 发病率的相关数据。本研究中描述的监测提供了有价值的信息，说明哪些血清型在流行，新的 HEV 出现情况以及与临床表现的关系。
