使用黏附性肿瘤细胞培养系统检测胃癌的化学敏感性

Chemosensitivity of gastric-cancer using adhesive tumor-cell culture system.

作者信息

Kitamura M, Furukawa H, Kito T, Nashimoto A, Kunii Y, Okabayashi K, Nakajima T, Fukushima M, Yanagawa T

机构信息

DEPT SURG ADULT DIS CANC,OSAKA,JAPAN. AICHI CANC CTR,DEPT SURG,NAGOYA,AICHI 464,JAPAN. NIIGATA CANC CTR,DEPT SURG,NIIGATA,JAPAN. AICHI CANC CTR,DEPT INTERNAL MED,NAGOYA,AICHI 464,JAPAN. SRL INC,TOKYO,JAPAN. NATL SENDAI HOSP,DEPT SURG,SENDAI,JAPAN. CANC INST HOSP,DEPT SURG,TOKYO,JAPAN. TOKYO METROPOLITAN KOMAGOME HOSP,JAPANESE GASTR CANC SURG STUDY GRP,BUNKYO KU,TOKYO 113,JAPAN.

出版信息

Oncol Rep. 1995 Jan;2(1):27-31.

PMID:21597682

Abstract

A total of 91 specimens of surgically resected tumors from 80 patients with gastric cancer were assayed for chemosensitivity using an adhesive tumor cell culture system (Life Trac ATCCS assay). Seventy-eight specimens of 91 had sufficient number of cells to grow in culture and 64 (82%) were evaluable excluding 8 low growth and 6 fungus contaminations. Cells (3x10(3)/ml/well) were cultured for 14 days and exposed to drugs on days 3-8. The growing cells were confirmed as cancer cells by immunohistochemical staining using monoclonal antibody to cytokeratin, epithelial membrane antigen and vimentin. IC90 value against (ADM, CDDP, CPM, 5-FU, MMC, MTX, VP-16, CBDCA and MMC+5-FU+MTX) was determined and population distribution of IC90 for each drug was obtained to serve as basic data for judging sensitivity. The 10th percentile of IC90 (mug/ml) was 0.01, 0.43, 1.23, 0.23, 0.01, 0.005, 0.14, 1.56 and 0.009+0.05+0.003 and median of IC90 was 0.02, 0.99, 2.31, 0.30, 0.06, 0.01, 0.39, 3.19 and 0.02+0.10+0.005, respectively. Population distribution of IC90 against each drug showed a specific pattern. Profiles of IC90 against various anticancer drugs differed in individual patients. Chemosensitivity of lymph node metatases seemed to be more resistant than that of their primary tumors. The ATTCS test was found to be useful as a sensitivity test for anti-cancer agents because of its reliability and excellent quantification.

摘要

使用粘附肿瘤细胞培养系统（Life Trac ATCCS检测法）对80例胃癌患者手术切除的91个肿瘤标本进行化疗敏感性检测。91个标本中有78个含有足够数量可在培养中生长的细胞，排除8个生长缓慢和6个真菌污染标本后，64个（82%）标本可用于评估。细胞（3×10³/ml/孔）培养14天，在第3至8天暴露于药物。使用抗细胞角蛋白、上皮膜抗原和波形蛋白的单克隆抗体通过免疫组织化学染色确认生长的细胞为癌细胞。测定了对（阿霉素、顺铂、环磷酰胺、5-氟尿嘧啶、丝裂霉素、甲氨蝶呤、依托泊苷、卡铂和丝裂霉素+5-氟尿嘧啶+甲氨蝶呤）的IC90值，并获得每种药物IC90的总体分布情况，作为判断敏感性的基础数据。IC90（μg/ml）的第10百分位数分别为0.01、0.43、1.23、0.23、0.01、0.005、0.14、1.56和0.009 + 0.05 + 0.003，IC90的中位数分别为0.02、0.99、2.31、0.30、0.06、0.01、0.39、3.19和0.02 + 0.10 + 0.005。每种药物IC90的总体分布呈现特定模式。不同患者对各种抗癌药物的IC90曲线不同。淋巴结转移灶的化疗敏感性似乎比其原发肿瘤更具耐药性。由于其可靠性和出色的定量性，发现ATTCS检测可作为抗癌药物敏感性检测方法。