Regulation of cll by interleukin-10 - role of antisense IL-10.

The role of IL-10 on the in vitro growth of B cells from patients with B-chronic lymphocytic leukemia (CLL) was investigated. Previous work on a murine model of CLL demonstrated that the malignant B-l cells produce significantly higher levels of IL-10 mRNA than normal B-1 or B cells and antisense oligodeoxynucleotides specific for IL-10 mRNA inhibited their growth. In the present study, peripheral blood cells from CLL patients were found to be varied in the amount of IL-10 mRNA present. Several CLL samples underwent apoptosis in response to culturing in the presence of antisense IL-10. There was a correlation between the levels of IL-IO mRNA and the sensitivity to growth inhibition by antisense IL-10. This may indicate that, antisense IL-10 inhibits cell growth in a sub-population of CLL in which IL-10 is an autocrine cytokine.