School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 May;28(5):619-26. doi: 10.1080/19440049.2011.563366.
Ronidazole was used as the starting material to prepare an immunogen and coating antigen. An anti-nitroimidazole monoclonal antibody was produced and an indirect competitive ELISA was established to detect nitroimidazole compounds in food products. The IC(50) values were determined to be 0.20 ng/ml for metronidazole, 4.0 ng/ml for tinidazole, 0.17 ng/ml for dimetridazole and 0.24 ng/ml for ornidazole. Considering that nitroimidazoles were commonly used as veterinary drugs, nitroimidazole residues in food products of animal origin were detected by the method. The coefficient of variation for nitroimidazoles determination in contaminated chicken, chicken liver and shrimp were all <14% and the recovery rate was in the range 74.0-90.6%. The results proved that the developed method was successful in detecting nitroimidazoles in food products.
罗硝唑被用作制备免疫原和包被抗原的起始原料。制备出一种抗硝基咪唑类单克隆抗体，并建立了间接竞争 ELISA 法用于检测食品中的硝基咪唑类化合物。米诺环素、替硝唑、二甲硝唑和奥硝唑的 IC50 值分别为 0.20ng/ml、4.0ng/ml、0.17ng/ml 和 0.24ng/ml。鉴于硝基咪唑类化合物通常被用作兽药，因此采用该方法检测动物源食品中的硝基咪唑类化合物残留。在受污染的鸡肉、鸡肝和虾中，硝基咪唑类化合物的测定变异系数均<14%，回收率在 74.0-90.6%范围内。结果表明，所建立的方法成功地用于检测食品中的硝基咪唑类化合物。
