高效液相色谱法测定家禽组织、血清和蛋类中的硝基咪唑残留量。

Determination of nitroimidazole residues in poultry tissues, serum and eggs by high-performance liquid chromatography.

作者信息

Semeniuk S, Posyniak A, Niedzielska J, Zmudzki J

机构信息

Department of Pharmacology and Toxicology, National Veterinary Research Institute, Pulawy, Poland.

出版信息

Biomed Chromatogr. 1995 Sep-Oct;9(5):238-42. doi: 10.1002/bmc.1130090510.

DOI:10.1002/bmc.1130090510

PMID:8593426

Abstract

A simple high-performance liquid chromatographic (HPLC) method was developed for determination of dimetridazole and metronidazole residues in poultry muscle, liver, serum and eggs. The drug residues were extracted from tissues and egg samples with acetonitrile followed by solid-phase extraction clean-up and HPLC analysis with photodiode array detector. Serum samples were treated with trichloroacetic acid, centrifuged and analysed without further clean-up. The detection limits were 2 ng/g and 5 ng/mL of analysed tissue (egg) and serum samples, respectively. Average recoveries for spike levels of 10 and 20 ng/g ranged from 82 to 94%, and coefficients of variation were below 10%.

摘要

建立了一种简单的高效液相色谱（HPLC）法，用于测定家禽肌肉、肝脏、血清和鸡蛋中的二甲硝唑和甲硝唑残留量。药物残留用乙腈从组织和鸡蛋样品中提取，然后进行固相萃取净化，并采用光电二极管阵列检测器进行HPLC分析。血清样品用三氯乙酸处理，离心后无需进一步净化即可进行分析。分析的组织（鸡蛋）和血清样品的检测限分别为2 ng/g和5 ng/mL。加标水平为10和20 ng/g时的平均回收率为82%至94%，变异系数低于10%。