Liu Chengwei, Xu Qin, Zhang Dan, Lu Xin, Zhao Shulin
College of Basic Medicine, Guilin Medical University, Guilin 541004, China.
Se Pu. 2011 Feb;29(2):157-61. doi: 10.3724/sp.j.1123.2011.00157.
A method was developed for the direct determination of netilmicin in rat serum using high performance liquid chromatography and resonance Rayleigh scattering (RRS). The separation was carried out on a C18 column with the isocratic elution of the mixture of 20 mmol/L sodium acetate aqueous solution and methanol containing 0.22% trifluoroacetic acid (92:8, v/v). With the molecular recognition probe of pontamine sky blue, netilmicin formed an ion-association complex and enhanced the intensity of RRS. The RRS signal was detected by a commercial fluorescence detector at 365 nm of both excitation wavelength (lambda ex) and emission wavelength (lambda em). Under the optimized conditions, the limit of detection (LOD, S/N = 3) for the netilmicin was 0.7 mg/L. With the internal standard (IS) of tobramycin, a linear calibration curve ranged from 1.2 mg/L to 30 mg/L was obtained. The presented method can be used for the pharmacokinetics study of netilmicin in rat serum, and can be a new choice for the determination of aminoglycoside antibiotics in biological samples.
建立了一种采用高效液相色谱法和共振瑞利散射（RRS）直接测定大鼠血清中奈替米星的方法。分离在C18柱上进行，以含0.22%三氟乙酸的20 mmol/L醋酸钠水溶液与甲醇的混合液（92∶8，v/v）等度洗脱。在达旦黄分子识别探针作用下，奈替米星形成离子缔合物，使RRS强度增强。采用商用荧光检测器在激发波长（λex）和发射波长（λem）均为365 nm处检测RRS信号。在优化条件下，奈替米星的检测限（LOD，S/N = 3）为0.7 mg/L。以内标妥布霉素得到了1.2 mg/L至30 mg/L的线性校准曲线。所提出的方法可用于大鼠血清中奈替米星的药代动力学研究，可为生物样品中氨基糖苷类抗生素的测定提供新的选择。
