[铜绿假单胞菌外毒素A重组无毒形式的免疫生物学特性]
[Immunobiological properties of recombinant atoxic forms of the Pseudomonas aeruginosa exotoxin A].
作者信息
Isakov M A, Soldatenkova A S, Kaloshin A A, Mikhaĭlova N A
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 2011 Mar-Apr(2):37-42.
AIM
Evaluation of immunobiological properties of recombinant atoxic forms of the Pseudomonas aeruginosa exotoxin. MATERIALS AND METHODS. 3 recombinant atoxic forms of the P. aeruginosa exotoxin A were produced and studied: aTox1, consisting only of exotoxin A domain 1; aTox1,2, consisting of domain 1 and 2; and aTox1,2,delta3, consisting of both domain 1 and 2, and part of domain 3.
RESULTS
aToxl,2 and aTox1,2,delta3 had distinctive antigenic properties. Formulations based on these recombinant proteins were immunogenic and protected animals from exotoxin A in experimental conditions.
CONCLUSION
These results maybe used to construct direct-action immunobiological formulations.
目的
评估铜绿假单胞菌外毒素重组无毒形式的免疫生物学特性。材料与方法。制备并研究了3种铜绿假单胞菌外毒素A的重组无毒形式：aTox1，仅由外毒素A结构域1组成；aTox1,2，由结构域1和2组成；以及aTox1,2,delta3，由结构域1和2以及部分结构域3组成。
结果
aTox1,2和aTox1,2,delta3具有独特的抗原特性。基于这些重组蛋白的制剂具有免疫原性，并在实验条件下保护动物免受外毒素A的侵害。
结论
这些结果可用于构建直接作用的免疫生物学制剂。
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