铜绿假单胞菌外毒素A重组无毒形式的制备。
Preparation of recombinant atoxic form of exotoxin A from Pseudomonas aeruginosa.
作者信息
Kaloshin A A, Isakov M A, Mikhailova N A, Vertiev Ju V
机构信息
I. I. Mechnikov Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow, Russia.
出版信息
Bull Exp Biol Med. 2013 Jan;154(3):346-50. doi: 10.1007/s10517-013-1947-1.
PMID:23484197
Abstract
Nucleotide sequences encoding full-length protein of Pseudomonas aeruginosa exotoxin A and its atoxic form were cloned and expressed in Escherichia coli cells. Purified recombinant exotoxin and immune rabbit sera protected mice from exotoxin A.
摘要
编码铜绿假单胞菌外毒素A全长蛋白及其无毒形式的核苷酸序列被克隆并在大肠杆菌细胞中表达。纯化的重组外毒素和免疫兔血清可保护小鼠免受外毒素A的侵害。
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