Kaloshin A A, Isakov M A, Mikhailova N A, Vertiev Ju V
I. I. Mechnikov Institute of Vaccines and Sera, Russian Academy of Medical Sciences, Moscow, Russia.
Bull Exp Biol Med. 2013 Jan;154(3):346-50. doi: 10.1007/s10517-013-1947-1.
Nucleotide sequences encoding full-length protein of Pseudomonas aeruginosa exotoxin A and its atoxic form were cloned and expressed in Escherichia coli cells. Purified recombinant exotoxin and immune rabbit sera protected mice from exotoxin A.
编码铜绿假单胞菌外毒素A全长蛋白及其无毒形式的核苷酸序列被克隆并在大肠杆菌细胞中表达。纯化的重组外毒素和免疫兔血清可保护小鼠免受外毒素A的侵害。
