人红细胞中环磷酸腺苷依赖性蛋白激酶的区室化
Compartmentalization of cyclic AMP-dependent protein kinases in human erythrocytes.
作者信息
Dreyfuss G, Schwartz K J, Blout E R
出版信息
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5926-30. doi: 10.1073/pnas.75.12.5926.
Abstract
The human erythrocyte contains two types of cyclic AMP-dependent protein kinase. The membrane-associated protein kinase holoenzyme can be released intact by hypotonic treatment at alkaline pH. Chromatography on DEAE-cellulose and molecular weight determinations demonstrate that this enzyme is a type I cyclic AMP-dependent protein kinase, while the predominant cyclic AMP-dependent protein kinase found in the cytoplasm is type II. The catalytic subunits of the two types of kinase found in the erythrocyte are identical, but the regulatory subunits, which distinguish the two types of kinase, determine their localization within the cell. It is proposed that the regulatory subunit of the type I enzyme, in addition to binding the catalytic subunit, interacts specifically with one or more membrane proteins and that this interaction may serve to position the kinase in preferential proximity to protein substrates.
摘要
人类红细胞含有两种环磷酸腺苷（cAMP）依赖性蛋白激酶。膜相关蛋白激酶全酶在碱性pH下经低渗处理可完整释放。通过DEAE - 纤维素色谱法和分子量测定表明，这种酶是I型cAMP依赖性蛋白激酶，而在细胞质中发现的主要的cAMP依赖性蛋白激酶是II型。红细胞中发现的两种激酶的催化亚基是相同的，但区分这两种激酶的调节亚基决定了它们在细胞内的定位。有人提出，I型酶的调节亚基除了与催化亚基结合外，还与一种或多种膜蛋白特异性相互作用，这种相互作用可能有助于将激酶定位在优先靠近蛋白质底物的位置。
相似文献
1
Compartmentalization of cyclic AMP-dependent protein kinases in human erythrocytes.人红细胞中环磷酸腺苷依赖性蛋白激酶的区室化
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5926-30. doi: 10.1073/pnas.75.12.5926.
2
3
Characterization of the protein kinase activities of human platelet supernatant and particulate fractions.人血小板上清液和颗粒组分的蛋白激酶活性表征
Biochem J. 1984 Mar 1;218(2):285-94. doi: 10.1042/bj2180285.
4
The effects of triethyltin bromide on red cell and brain cyclic AMP-dependent protein kinases.溴化三乙锡对红细胞和脑环磷酸腺苷依赖性蛋白激酶的影响。
J Biol Chem. 1983 Sep 25;258(18):11384-90.
5
Purification and characterization of a catalytic subunit of an adenosine 3':5'-monophosphate-dependent protein kinase from human erythrocyte membranes.人红细胞膜中依赖3':5'-单磷酸腺苷的蛋白激酶催化亚基的纯化与特性分析
J Biochem. 1981 Jan;89(1):1-11. doi: 10.1093/oxfordjournals.jbchem.a133169.
6
Phosphorylation of rabbit and human erythrocyte membranes by soluble adenosine 3':5'-monophosphate-dependent and -independent protein kinases.
J Biol Chem. 1977 Jan 10;252(1):102-9.
7
In vitro phosphorylation of the red blood cell cytoskeleton complex by cyclic AMP-dependent protein kinase from erythrocyte membrane.红细胞膜中环磷酸腺苷依赖性蛋白激酶对红细胞细胞骨架复合物的体外磷酸化作用。
Biochim Biophys Acta. 1981 Sep 21;647(1):1-6. doi: 10.1016/0005-2736(81)90289-3.
8
Human erythrocyte proteins associated with adenosine 3',5'-cyclic monophosphate action.与3',5'-环磷酸腺苷作用相关的人红细胞蛋白。
Enzyme. 1978;23(2):73-83. doi: 10.1159/000458555.
9
Adenosine cyclic 3',5'-monophosphate-dependent protein kinase from human platelets.来自人血小板的环磷腺苷依赖蛋白激酶
Biochim Biophys Acta. 1976 Jan 23;422(1):60-72. doi: 10.1016/0005-2744(76)90008-5.
10
Localization of catalytic and regulatory subunits of cyclic AMP-dependent protein kinases in mitochondria from various rat tissues.环磷酸腺苷依赖性蛋白激酶的催化亚基和调节亚基在大鼠不同组织线粒体中的定位
Biochem J. 1990 Aug 15;270(1):181-8. doi: 10.1042/bj2700181.
引用本文的文献
1
Inhibition of ATP release from erythrocytes: a role for EPACs and PKC.抑制红细胞中 ATP 的释放：EPACs 和 PKC 的作用。
Microcirculation. 2011 Feb;18(2):128-35. doi: 10.1111/j.1549-8719.2010.00073.x.
2
Protein kinases A and C regulate receptor-mediated increases in cAMP in rabbit erythrocytes.蛋白激酶 A 和 C 调节兔红细胞中受体介导的 cAMP 增加。
Am J Physiol Heart Circ Physiol. 2010 Feb;298(2):H587-93. doi: 10.1152/ajpheart.00975.2009. Epub 2009 Dec 11.
3
Plasmodium falciparum regulatory subunit of cAMP-dependent PKA and anion channel conductance.恶性疟原虫环磷酸腺苷依赖性蛋白激酶A的调节亚基与阴离子通道电导
PLoS Pathog. 2008 Feb 8;4(2):e19. doi: 10.1371/journal.ppat.0040019.
4
Interaction of Ca2+ and protein phosphorylation in the rabbit platelet release reaction.兔血小板释放反应中钙离子与蛋白质磷酸化的相互作用。
J Clin Invest. 1980 Feb;65(2):242-55. doi: 10.1172/JCI109666.
5
Defining erythrocyte internal labeling by phosphorylation.通过磷酸化定义红细胞内部标记。
Proc Natl Acad Sci U S A. 1984 May;81(9):2767-71. doi: 10.1073/pnas.81.9.2767.
6
Characterization of the protein kinase activities of human platelet supernatant and particulate fractions.人血小板上清液和颗粒组分的蛋白激酶活性表征
Biochem J. 1984 Mar 1;218(2):285-94. doi: 10.1042/bj2180285.
7
Characterization and comparison of the soluble and membrane-bound cyclic AMP-dependent protein kinase from swine kidney.猪肾可溶性和膜结合环磷酸腺苷依赖性蛋白激酶的特性及比较
Mol Cell Biochem. 1983;50(2):157-71. doi: 10.1007/BF00285641.
8
Changes in cyclic AMP-dependent protein kinases during inhibition of mastocytoma cell growth by dibutyryl cyclic AMP.二丁酰环磷腺苷抑制肥大细胞瘤细胞生长过程中环磷腺苷依赖性蛋白激酶的变化
Mol Cell Biochem. 1982 Apr 2;43(3):183-90. doi: 10.1007/BF00223009.
9
Regulation of ornithine decarboxylase activity by corticotropin in adrenocortical tumor cell clones: roles of cyclic AMP and cyclic AMP-dependent protein kinase.促肾上腺皮质激素对肾上腺皮质肿瘤细胞克隆中鸟氨酸脱羧酶活性的调节：环磷酸腺苷和环磷酸腺苷依赖性蛋白激酶的作用
Proc Natl Acad Sci U S A. 1980 May;77(5):2767-71. doi: 10.1073/pnas.77.5.2767.
10
Deficiency of cyclic AMP-dependent protein kinases in human psoriasis.人类银屑病中依赖环磷酸腺苷的蛋白激酶缺乏症。
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5272-6. doi: 10.1073/pnas.83.14.5272.
本文引用的文献
1
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
2
Molecular biology of cellular membranes with applications to immunology.细胞膜的分子生物学及其在免疫学中的应用
Adv Immunol. 1974;19(0):1-66. doi: 10.1016/s0065-2776(08)60251-5.
3
Phosphorylation of endogenous substrates by erythrocyte membrane protein kinases. II. Cyclic adenosine monophosphate-stimulated reactions.红细胞膜蛋白激酶对内源性底物的磷酸化作用。II. 环磷酸腺苷刺激的反应。
Biochemistry. 1974 Dec 31;13(27):5514-21. doi: 10.1021/bi00724a010.
4
Preparation of impermeable ghosts and inside-out vesicles from human erythrocyte membranes.从人红细胞膜制备不透性血影和外翻小泡。
Methods Enzymol. 1974;31:172-80. doi: 10.1016/0076-6879(74)31019-1.
5
Protein kinases.蛋白激酶
Curr Top Cell Regul. 1972;5:99-133.
6
The role of cyclic AMP in the phosphorylation of proteins in human erythrocyte membranes.
Biochem Biophys Res Commun. 1973 Jan 23;50(2):421-9. doi: 10.1016/0006-291x(73)90857-7.
7
Erythrocyte protein phosphorylation.红细胞蛋白磷酸化
J Biol Chem. 1973 Feb 25;248(4):1408-11.
8
Cyclic adenosine 3',5'-monophosphate-dependent protein kinase of human erythrocyte membranes.人红细胞膜的环腺苷酸依赖性蛋白激酶
J Biol Chem. 1972 Oct 10;247(19):6135-9.
9
10
A protein binding assay for adenosine 3':5'-cyclic monophosphate.一种用于检测3':5'-环磷酸腺苷的蛋白质结合测定法。
Proc Natl Acad Sci U S A. 1970 Sep;67(1):305-12. doi: 10.1073/pnas.67.1.305.
Zotero 插件