Evans J, Smart J, Airey P, Ralph R
Mol Cell Biochem. 1982 Apr 2;43(3):183-90. doi: 10.1007/BF00223009.
Inhibition of growth of PY815 mouse mastocytoma cells in vitro by N6,O2'-dibutyryladenosine 3',5' cyclic monophosphate (DB cyclic AMP) was accompanied by increases in intracellular cyclic AMP and histamine and minor changes in cytosolic cyclic AMP-dependent histone kinase activity. However, DEAE-cellulose chromatography revealed substantial changes in the relative proportions of the principal cyclic AMP-dependent protein kinases and in free cyclic AMP-binding protein after DB cyclic AMP treatment. The activity of cytosolic cyclic AMP-dependent protein kinase type I (PKI) decreased relative to cyclic AMP-dependent protein kinase type II (PKII) and there was an increase in a cytosol cyclic AMP-binding protein with little associated protein kinase activity. The relative changes in activity of PKI, PKII and cyclic AMP binding protein after DB cyclic AMP treatment may reflect events important in the regulation of growth and differentiation of mast cells.
N6,O2'-二丁酰腺苷3',5'-环一磷酸（DB环磷腺苷）对PY815小鼠肥大细胞瘤细胞体外生长的抑制作用伴随着细胞内环磷腺苷和组胺的增加以及胞质环磷腺苷依赖性组蛋白激酶活性的微小变化。然而，DEAE-纤维素色谱分析显示，DB环磷腺苷处理后，主要的环磷腺苷依赖性蛋白激酶的相对比例以及游离环磷腺苷结合蛋白发生了显著变化。相对于环磷腺苷依赖性蛋白激酶II型（PKII），胞质环磷腺苷依赖性蛋白激酶I型（PKI）的活性降低，并且具有很少相关蛋白激酶活性的胞质环磷腺苷结合蛋白增加。DB环磷腺苷处理后PKI、PKII和环磷腺苷结合蛋白活性的相对变化可能反映了在肥大细胞生长和分化调节中重要的事件。
