Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.za della Scienza 2, 20126 Milano, Italy.
Carbohydr Res. 2011 Sep 6;346(12):1564-8. doi: 10.1016/j.carres.2011.04.044. Epub 2011 May 3.
An efficient method for the direct and covalent decoration of granules of nanostructured apatite with a sample monosaccharide is presented; the hydroxyapatite material was directly functionalised with a short azido-containing spacer arm, to which α-propargyl glucopyranoside has been chemoselectively ligated by Huisgen-type cycloaddition. The 'glycosylated' hydroxypatite was characterised by its ability to interact with glucose recognising lectins.
本文提出了一种将单糖样品直接和共价修饰纳米结构磷灰石颗粒的有效方法；通过羟基磷灰石材料直接功能化短的叠氮化物间隔臂，通过 Huisgen 型环加成反应，化学选择性地连接α-炔丙基吡喃葡萄糖苷。通过与识别葡萄糖的凝集素相互作用，对“糖基化”羟基磷灰石进行了表征。
