College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350108, People's Republic of China.
Int J Biol Macromol. 2011 Oct 1;49(3):281-8. doi: 10.1016/j.ijbiomac.2011.05.004. Epub 2011 May 11.
A tubular scaffold was fabricated by using electrospun polymer solution blends of pNSR32 (recombinant spider silk protein), PCL (polycaprolactone) and Gt (gelatin). The physicochemical properties and cytocompatibility of these scaffolds were investigated. Afterwards, the pNSR32/PCL/Gt tubular scaffold (inner diameter=3mm) showed high porosity of 86.2 ± 2.9%, pore size of 2423 ± 979nm and average fibre diameter of 166 ± 85nm. Water uptake and contact angle of the scaffolds reached 112.0 ± 4.4% and 45.7 ± 13.7°, respectively. SDRAECs (Sprague Dawley Rat Aortic Endothelial Cells) grew and proliferated well and phenotype could be maintained on the composite scaffolds after they had been cultured on the composite scaffolds for 7 days. Compared with pure PCL scaffolds a greater density of viable cells was seen on the composites, especially the pNSR32/PCL/Gt scaffolds.
采用静电纺丝聚合物溶液共混的方法制备管状支架，其中包含 pNSR32（重组蜘蛛丝蛋白）、PCL（聚己内酯）和 Gt（明胶）。研究了这些支架的物理化学性质和细胞相容性。结果表明，pNSR32/PCL/Gt 管状支架（内径=3mm）具有 86.2±2.9%的高孔隙率、2423±979nm 的孔径和 166±85nm 的平均纤维直径。支架的吸水率和接触角分别达到 112.0±4.4%和 45.7±13.7°。SDRAECs（Sprague Dawley Rat Aortic Endothelial Cells，SDRAECs）在复合支架上生长和增殖良好，在复合支架上培养 7 天后可维持其表型。与纯 PCL 支架相比，在复合材料上观察到更多的活细胞密度，尤其是 pNSR32/PCL/Gt 支架。
